Rabbit Polyclonal NCOR2/SMRT antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCOR2 aa 50-600.
View Alternative Names
CTG26, NCOR2, Nuclear receptor corepressor 2, N-CoR2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, T3 receptor-associating factor, SMRT, TRAC
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOR2/SMRT antibody (AB5802)
ab5802 labelling NCOR2/SMRT in human lung tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1 : 500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (AB5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (AB5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1 : 150 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (AB5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (AB5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1 : 150 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOR2/SMRT antibody (AB5802)
ab5802 labelling NCOR2/SMRT in mouse brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1 : 500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Reactivity data
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Supplementary information
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Biological function summary
This protein functions as part of a large multi-protein complex called the SMRT/N-CoR complex. It acts by binding to un-liganded nuclear receptors such as thyroid hormone receptor and retinoic acid receptor which leads to repression of target genes essential for diverse cellular processes. This regulatory action affects cell growth differentiation and metabolism implicating NCOR2's role in maintaining normal cellular functions.
Pathways
NCOR2 participates in key pathways such as the thyroid hormone signaling pathway and the retinoic acid pathway. These pathways are critical in managing metabolism and development. NCOR2 relates to proteins like histone deacetylases (HDACs) which are part of its repression complex and key to regulating transcription. In these pathways NCOR2's interaction with nuclear receptors directs the outcome of transcriptional activity necessary for proper physiological responses.
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Target data
Publications (6)
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The EMBO journal 43:4578-4603 PubMed39210147
2024
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Nature communications 14:2559 PubMed37137875
2023
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Nature 614:326-333 PubMed36599367
2023
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Nature communications 12:7045 PubMed34857762
2021
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Cell 176:182-197.e23 PubMed30595450
2019
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IP
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Mouse
The Journal of clinical investigation 117:719-29 PubMed17318262
2007
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