Rabbit Polyclonal NCOR2/SMRT antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCOR2 aa 50-600.
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- Nature communications 14:25592023The Fgf/Erf/NCoR1/2 repressive axis controls trophoblast cell fate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndreas Lackner,Michael Müller,Magdalena Gamperl,Delyana Stoeva,Olivia Langmann,Henrieta Papuchova,Elisabeth Roitinger,Gerhard Dürnberger,Richard Imre,Karl Mechtler,Paulina A LatosPubMed 37137875
- Nature communications 12:70452021Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiro Matsumura,Ryo Ito,Ayumu Yajima,Rei Yamaguchi,Toshiya Tanaka,Takeshi Kawamura,Kenta Magoori,Yohei Abe,Aoi Uchida,Takeshi Yoneshiro,Hiroyuki Hirakawa,Ji Zhang,Makoto Arai,Chaoran Yang,Ge Yang,Hiroki Takahashi,Hitomi Fujihashi,Ryo Nakaki,Shogo Yamamoto,Satoshi Ota,Shuichi Tsutsumi,Shin-Ichi Inoue,Hiroshi Kimura,Youichiro Wada,Tatsuhiko Kodama,Takeshi Inagaki,Timothy F Osborne,Hiroyuki Aburatani,Koichi Node,Juro SakaiPubMed 34857762
- Cell 176:182-197.e232019The Implication of Early Chromatin Changes in X Chromosome Inactivation.Applications:IPReactive species:MouseJan Jakub Żylicz,Aurélie Bousard,Kristina Žumer,Francois Dossin,Eusra Mohammad,Simão Teixeira da Rocha,Björn Schwalb,Laurène Syx,Florent Dingli,Damarys Loew,Patrick Cramer,Edith HeardPubMed 30595450
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NCOR2 aa 50-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y618
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Transcriptional corepressor that mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PubMed:10077563, PubMed:10097068, PubMed:18212045, PubMed:20812024, PubMed:22230954, PubMed:23911289). Acts by recruiting chromatin modifiers, such as histone deacetylases HDAC1, HDAC2 and HDAC3 (PubMed:22230954). Required to activate the histone deacetylase activity of HDAC3 (PubMed:22230954). Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival (PubMed:18212045, PubMed:23911289). Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Isoform 1. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Isoform 4. Isoform 1 and isoform 4 have different affinities for different nuclear receptors.
Alternative names
CTG26, NCOR2, Nuclear receptor corepressor 2, N-CoR2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, T3 receptor-associating factor, SMRT, TRAC
Recommended products
Rabbit Polyclonal NCOR2/SMRT antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCOR2 aa 50-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NCOR2 aa 50-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y618
- Purification technique
- Affinity purification Immunogen
- Specificity
ab5802 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human cells.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCOR2 also known as SMRT (Silencing Mediator for Retinoid and Thyroid receptors) is a nuclear receptor co-repressor important in gene regulation. It weighs around 270 kDa and expresses in various tissues including the brain liver and lungs. Mechanically NCOR2 interacts with nuclear receptors and other transcription factors to inhibit gene expression by facilitating the formation of a complex that deacetylates histones causing the chromatin structure to tighten and less transcription to happen.
- Biological function summary
This protein functions as part of a large multi-protein complex called the SMRT/N-CoR complex. It acts by binding to un-liganded nuclear receptors such as thyroid hormone receptor and retinoic acid receptor which leads to repression of target genes essential for diverse cellular processes. This regulatory action affects cell growth differentiation and metabolism implicating NCOR2's role in maintaining normal cellular functions.
- Pathways
NCOR2 participates in key pathways such as the thyroid hormone signaling pathway and the retinoic acid pathway. These pathways are critical in managing metabolism and development. NCOR2 relates to proteins like histone deacetylases (HDACs) which are part of its repression complex and key to regulating transcription. In these pathways NCOR2's interaction with nuclear receptors directs the outcome of transcriptional activity necessary for proper physiological responses.
- Associated diseases and disorders
Research links NCOR2 to various conditions including acute promyelocytic leukemia (APL) and metabolic disorders. In APL NCOR2 can form part of a fusion protein with retinoic acid receptor-alpha (RARα) disrupting normal gene regulation and promoting cancer. Furthermore its role in transcription repression connects NCOR2 to metabolic dysfunctions suggesting its potential involvement in obesity-related insulin resistance. This relationship is broadened through association with other co-repressor proteins like N-CoR emphasizing the importance of its regulatory function in health and disease.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOR2/SMRT antibody (ab5802)
ab5802 labelling NCOR2/SMRT in mouse brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOR2/SMRT antibody (ab5802)
ab5802 labelling NCOR2/SMRT in human lung tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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