Name: Anti-NCR1 antibody [EPR23097-35]
SKU: ab233558
Rating: 5 (2 reviews)

Rabbit Recombinant Monoclonal NCR1 antibody. Suitable for IHC-P, mIHC and reacts with Mouse, Rat samples. Cited in 9 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (AB233558), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	WB	ICC/IF	IHC-Fr	IHC-P	mIHC
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Tested	Tested
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Tested	Expected

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information Ncr1

Function

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

Alternative names

CD335, Ly94, Ncr1, Natural cytotoxicity triggering receptor 1, Activating receptor 1, Lymphocyte antigen 94, Natural killer cell p46-related protein, mAR-1, NK-p46, NKp46, mNKp46

Recommended products

Rabbit Recombinant Monoclonal NCR1 antibody. Suitable for IHC-P, mIHC and reacts with Mouse, Rat samples. Cited in 9 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23097-35
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NCR1 also known as NKp46 is a receptor mainly expressed on the surface of natural killer (NK) cells. Its primary role involves recognizing and binding to ligands present on target cells including tumor cells and virus-infected cells. NCR1 initiates the activation of NK cells leading to the destruction of these abnormal cells. The NCR1 protein has a molecular weight of approximately 46 kDa and is a part of the immunoglobulin superfamily. NCR1 is mainly expressed in the immune system specifically on NK cells which are an important component of the innate immune response.

Biological function summary

The function of NCR1 involves the recognition of stressed transformed or infected cells marking them for elimination by NK cells. It does not typically function as a part of a complex; rather it serves a somewhat autonomous role in cell signaling. However its interaction with other receptors on NK cells including DAP12 enhances its signaling capabilities. Through the activation of cascades NCR1 promotes the release of cytotoxic granules and the production of cytokines therefore mediating the immune response.

Pathways

NCR1 plays a significant role in the regulation of immune surveillance pathways. It is involved in the recognition and elimination pathways that modulate the immune response against infected or cancerous cells. NK cells with NCR1's involvement interact with receptors like NKG2D adjusting the NK cell's cytotoxic activity. These interactions coordinate the broader immune response important for innate immunity effectively bridging innate and adaptive immunity by influencing other immune cells.

Associated diseases and disorders

NCR1 has been implicated in cancer and infectious diseases. In cancer altered expression or dysfunction of NCR1 may contribute to the evasion of immune surveillance by tumor cells potentially leading to tumor progression. Studies have shown reduced NKp46 expression in some cancerous states linking it to poor prognosis. In infectious diseases NCR1 plays a role in targeting virus-infected cells and its dysfunction can result in reduced effectiveness in clearing viral infections. Interactions with viral envelope proteins can interfere with NCR1 activity exemplifying its importance in antiviral defense mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the rat spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the mouse spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Multiplex immunohistochemistry - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node staining of NCR1 with ab233558 at a 1/500 (1.062 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at 1/2000 ( 0.264 µg/ml) dilution.
Panel A: merged staining of anti-NCR1 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse lymph node.
Panel B: anti-NCR1 staining natural killer cells in mouse lymph node.
Panel C: anti-CD19 staining B lymphocytes in mouse lymph node..
Panel D: anti-CD3 staining T lymphocytes in mouse lymph node.
Nuclear DNA was labelled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab233558, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen staining of NCR1 with ab233558 at a 1/500 (1.062 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at 1/2000 ( 0.264 µg/ml) dilution.
Panel A: merged staining of anti-NCR1 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse spleen.
Panel B: Anti-NCR1 staining natural killer cells in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labelled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab233558, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining CD94 with Anti-CD94 antibody[EPR26343-41] ab322460 at a 1:100 (5.07 ug/ml) dilution, ab233558 anti-NCR1 used at 1:500 (1.114 ug/ml) dilution.
Panel A: merged staining of anti-CD94 (green; Opal™520), anti-NCR1 (magenta; Opal™570) on mouse spleen.

Panel B: anti-CD94 staining natural killer (NK) cells in mouse spleen.

Panel C: anti-NCR11 staining natural killer (NK) cells in mouse spleen.

Panel D: Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-CD94 antibody[EPR26343-41] ab322460 and ab233558 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This image is courtesy of an anonymous customer review
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of 10% NBF-fixed mouse spleen tissue. Stained with ab233558 at 1/200 dilution.
Secondary antibody used was HRP Goat anti-Rabbit IgG at 1/250 dilution. Blocking was done with 15% serum for 1 hour at room temperature. The sample was incubated with the primary antibody and TBS for 18 hours. Antigen retrieval method was heat mediated, Tris/EDTA pH 9.0.
Multiplex immunohistochemistry - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus staining of NCR1 with ab233558 at a 1/500 (1.062 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at 1/2000 ( 0.264 µg/ml) dilution.
Panel A: merged staining of anti-NCR1 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse thymus.
Panel B: anti-NCR1 staining natural killer cells in mouse thymus.
Panel C: anti-CD19 staining B lymphocytes in mouse thymus.
Panel D: anti-CD3 staining T lymphocytes in mouse thymus.
Nuclear DNA was labelled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab233558, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.