Mouse Recombinant Monoclonal NCR1 antibody. Carrier free. Suitable for IHC-P, ELISA and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173634.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164772.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181237.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176374.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517)")
![ELISA - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--elisa-img165440.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (AB283517)")
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | Flow Cyt | ELISA | WB | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended |
| Rat | Tested | Not recommended | Expected | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Alternative names
CD335, Ly94, Ncr1, Natural cytotoxicity triggering receptor 1, Activating receptor 1, Lymphocyte antigen 94, Natural killer cell p46-related protein, mAR-1, NK-p46, NKp46, mNKp46
Recommended products
Mouse Recombinant Monoclonal NCR1 antibody. Carrier free. Suitable for IHC-P, ELISA and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- mNCR1.05
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab283517 is the carrier-free version of Anti-NCR1 antibody [mNCR1.05] ab283505.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCR1 also known as NKp46 is a receptor mainly expressed on the surface of natural killer (NK) cells. Its primary role involves recognizing and binding to ligands present on target cells including tumor cells and virus-infected cells. NCR1 initiates the activation of NK cells leading to the destruction of these abnormal cells. The NCR1 protein has a molecular weight of approximately 46 kDa and is a part of the immunoglobulin superfamily. NCR1 is mainly expressed in the immune system specifically on NK cells which are an important component of the innate immune response.
- Biological function summary
The function of NCR1 involves the recognition of stressed transformed or infected cells marking them for elimination by NK cells. It does not typically function as a part of a complex; rather it serves a somewhat autonomous role in cell signaling. However its interaction with other receptors on NK cells including DAP12 enhances its signaling capabilities. Through the activation of cascades NCR1 promotes the release of cytotoxic granules and the production of cytokines therefore mediating the immune response.
- Pathways
NCR1 plays a significant role in the regulation of immune surveillance pathways. It is involved in the recognition and elimination pathways that modulate the immune response against infected or cancerous cells. NK cells with NCR1's involvement interact with receptors like NKG2D adjusting the NK cell's cytotoxic activity. These interactions coordinate the broader immune response important for innate immunity effectively bridging innate and adaptive immunity by influencing other immune cells.
- Associated diseases and disorders
NCR1 has been implicated in cancer and infectious diseases. In cancer altered expression or dysfunction of NCR1 may contribute to the evasion of immune surveillance by tumor cells potentially leading to tumor progression. Studies have shown reduced NKp46 expression in some cancerous states linking it to poor prognosis. In infectious diseases NCR1 plays a role in targeting virus-infected cells and its dysfunction can result in reduced effectiveness in clearing viral infections. Interactions with viral envelope proteins can interfere with NCR1 activity exemplifying its importance in antiviral defense mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173634.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)
This data was developed using Anti-NCR1 antibody [mNCR1.05] ab283505 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NCR1 with Anti-NCR1 antibody [mNCR1.05] ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in mouse spleen.The section was incubated with Anti-NCR1 antibody [mNCR1.05] ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164772.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)
This data was developed using Anti-NCR1 antibody [mNCR1.05] ab283505 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling NCR1 with Anti-NCR1 antibody [mNCR1.05] ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in mouse colon.The section was incubated with Anti-NCR1 antibody [mNCR1.05] ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181237.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)
This data was developed using Anti-NCR1 antibody [mNCR1.05] ab283505 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling NCR1 with Anti-NCR1 antibody [mNCR1.05] ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in rat spleen.The section was incubated with Anti-NCR1 antibody [mNCR1.05] ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176374.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)
This data was developed using Anti-NCR1 antibody [mNCR1.05] ab283505 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling NCR1 with Anti-NCR1 antibody [mNCR1.05] ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in rat lung.The section was incubated with Anti-NCR1 antibody [mNCR1.05] ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![ELISA - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517), expandable thumbnail](https://content.abcam.com/products/images/ncr1-antibody-mncr105-bsa-and-azide-free-ab283517--elisa-img165440.jpg "ELISA - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)")
ELISA - Anti-NCR1 antibody [mNCR1.05] - BSA and Azide free (ab283517)
This data was developed using Anti-NCR1 antibody [mNCR1.05] ab283505 the same antibody clone in a different buffer formulation.
ELISA analysis using Anti-NCR1 antibody [mNCR1.05] ab283505 at a range of 0-500 ng/ml followed by a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）at 1/1000 dilution. Antigen concentration: 1000 ng/ml.
Antigens: Mouse NKp46/NCR1 Fc Chimera Protein.
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