AB233808

Anti-NCR1/NKp46 antibody [2B11E12]

Name: Anti-NCR1/NKp46 antibody [2B11E12] (ab233808) | Abcam
Brand: Abcam Limited
SKU: AB233808
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal NCR1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NCR1.

View Alternative Names

CD335, LY94, NCR1, Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46

3 Images

Flow Cytometry - Anti-NCR1/NKp46 antibody [2B11E12] (AB233808)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-NCR1/NKp46 antibody [2B11E12] (AB233808)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling NCR1 using ab233808 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-NCR1/NKp46 antibody [2B11E12] (AB233808)

Expected MW is 52.8 kDa.

All lanes:

Western blot - Anti-NCR1/NKp46 antibody [2B11E12] (ab233808) at 1/500 dilution

All lanes:

Recombinant human NCR1 protein

Predicted band size: 34 kDa

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Supplier Data

Western blot - Anti-NCR1/NKp46 antibody [2B11E12] (AB233808)

All lanes:

Western blot - Anti-NCR1/NKp46 antibody [2B11E12] (ab233808) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

NCR1 (aa 22-258)-hIgGFc transfected HEK-293 cell lysate.

Predicted band size: 34 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2B11E12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NCR1.

O76036

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCR1 also known as NKp46 is a receptor mainly expressed on the surface of natural killer (NK) cells. Its primary role involves recognizing and binding to ligands present on target cells including tumor cells and virus-infected cells. NCR1 initiates the activation of NK cells leading to the destruction of these abnormal cells. The NCR1 protein has a molecular weight of approximately 46 kDa and is a part of the immunoglobulin superfamily. NCR1 is mainly expressed in the immune system specifically on NK cells which are an important component of the innate immune response.

Biological function summary

The function of NCR1 involves the recognition of stressed transformed or infected cells marking them for elimination by NK cells. It does not typically function as a part of a complex; rather it serves a somewhat autonomous role in cell signaling. However its interaction with other receptors on NK cells including DAP12 enhances its signaling capabilities. Through the activation of cascades NCR1 promotes the release of cytotoxic granules and the production of cytokines therefore mediating the immune response.

Pathways

NCR1 plays a significant role in the regulation of immune surveillance pathways. It is involved in the recognition and elimination pathways that modulate the immune response against infected or cancerous cells. NK cells with NCR1's involvement interact with receptors like NKG2D adjusting the NK cell's cytotoxic activity. These interactions coordinate the broader immune response important for innate immunity effectively bridging innate and adaptive immunity by influencing other immune cells.

NCR1 has been implicated in cancer and infectious diseases. In cancer altered expression or dysfunction of NCR1 may contribute to the evasion of immune surveillance by tumor cells potentially leading to tumor progression. Studies have shown reduced NKp46 expression in some cancerous states linking it to poor prognosis. In infectious diseases NCR1 plays a role in targeting virus-infected cells and its dysfunction can result in reduced effectiveness in clearing viral infections. Interactions with viral envelope proteins can interfere with NCR1 activity exemplifying its importance in antiviral defense mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

See full target information NCR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:2779-2796.e10 PubMed35675814

2022

AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Cho,Elizabeth T Abshire,Maximilian W Popp,Christoph Pröschel,Joshua L Schwartz,Gene W Yeo,Lynne E Maquat

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