AB199128

Anti-NCR1/NKp46 antibody

Name: Anti-NCR1/NKp46 antibody (ab199128) | Abcam
Brand: Abcam Limited
SKU: AB199128
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal NCR1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NCR1.

View Alternative Names

CD335, LY94, NCR1, Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46

4 Images

Supplier Data

Western blot - Anti-NCR1/NKp46 antibody (AB199128)

Gel : 8% SDS-PAGE.

All lanes:

Western blot - Anti-NCR1/NKp46 antibody (ab199128) at 1/450 dilution

All lanes:

Mouse kidney tissue lysate at 40 µg

Predicted band size: 34 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody (AB199128)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody (AB199128)

Immunofluorescence of Human esophagus cancer labeling NCR1 with ab199128 at 1 : 20. A FITC conjugated antibody was used as a secondry.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody (AB199128)

Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labeling NCR1 with ab199128 at 1/20 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody (AB199128)

Immunofluorescence of Human lung cancer labeling NCR1 with ab199128 at 1 : 20. A FITC conjugated antibody was used as a secondry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NCR1. The exact immunogen used to generate this antibody is proprietary information.

O76036

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCR1 also known as NKp46 is a receptor mainly expressed on the surface of natural killer (NK) cells. Its primary role involves recognizing and binding to ligands present on target cells including tumor cells and virus-infected cells. NCR1 initiates the activation of NK cells leading to the destruction of these abnormal cells. The NCR1 protein has a molecular weight of approximately 46 kDa and is a part of the immunoglobulin superfamily. NCR1 is mainly expressed in the immune system specifically on NK cells which are an important component of the innate immune response.

Biological function summary

The function of NCR1 involves the recognition of stressed transformed or infected cells marking them for elimination by NK cells. It does not typically function as a part of a complex; rather it serves a somewhat autonomous role in cell signaling. However its interaction with other receptors on NK cells including DAP12 enhances its signaling capabilities. Through the activation of cascades NCR1 promotes the release of cytotoxic granules and the production of cytokines therefore mediating the immune response.

Pathways

NCR1 plays a significant role in the regulation of immune surveillance pathways. It is involved in the recognition and elimination pathways that modulate the immune response against infected or cancerous cells. NK cells with NCR1's involvement interact with receptors like NKG2D adjusting the NK cell's cytotoxic activity. These interactions coordinate the broader immune response important for innate immunity effectively bridging innate and adaptive immunity by influencing other immune cells.

NCR1 has been implicated in cancer and infectious diseases. In cancer altered expression or dysfunction of NCR1 may contribute to the evasion of immune surveillance by tumor cells potentially leading to tumor progression. Studies have shown reduced NKp46 expression in some cancerous states linking it to poor prognosis. In infectious diseases NCR1 plays a role in targeting virus-infected cells and its dysfunction can result in reduced effectiveness in clearing viral infections. Interactions with viral envelope proteins can interfere with NCR1 activity exemplifying its importance in antiviral defense mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

See full target information NCR1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 11: PubMed37072348

2023

Intratumoral tertiary lymphoid structure (TLS) maturation is influenced by draining lymph nodes of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miao He,Qihua He,Xiuyu Cai,Jun Liu,Hongshen Deng,Feng Li,Ran Zhong,Yi Lu,Haoxin Peng,Xiangrong Wu,Zisheng Chen,Shen Lao,Caichen Li,Jianfu Li,Jianxing He,Wenhua Liang

Proceedings of the National Academy of Sciences of 117:29823-29831 PubMed33168738

2020

Excess of the NF-ĸB p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yelena Kravtsova-Ivantsiv,Gilad Goldhirsh,Alexandra Ivantsiv,Ofer Ben Itzhak,Yong Tae Kwon,Eli Pikarsky,Aaron Ciechanover

Life science alliance 2: PubMed30936185

2019

Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Matsuda,Rintaro Ono,Tomonori Iyoda,Takaho Endo,Makoto Iwasaki,Mariko Tomizawa-Murasawa,Yoriko Saito,Akiko Kaneko,Kanako Shimizu,Daisuke Yamada,Narumi Ogonuki,Takashi Watanabe,Manabu Nakayama,Yoko Koseki,Fuyuko Kezuka-Shiotani,Takanori Hasegawa,Hiromasa Yabe,Shunichi Kato,Atsuo Ogura,Leonard D Shultz,Osamu Ohara,Masaru Taniguchi,Haruhiko Koseki,Shin-Ichiro Fujii,Fumihiko Ishikawa

International journal of molecular sciences 18: PubMed29137183

2017

Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqin Qiu,Zongyong Jiang,Shenglan Hu,Li Wang,Xianyong Ma,Xuefen Yang

Oncotarget 8:54775-54787 PubMed28903381

2017

Tumor location impacts immune response in mouse models of colon cancer.

Applications

IHC-P

Species

Mouse

Xianda Zhao,Lihua Li,Timothy K Starr,Subbaya Subramanian

Cancer science 107:882-9 PubMed27145494

2016

A prognostic risk model for patients with triple negative breast cancer based on stromal natural killer cells, tumor-associated macrophages and growth-arrest specific protein 6.

Applications

IHC-P

Species

Human

Wenjing Tian,Le Wang,Lili Yuan,Wenming Duan,Wenhui Zhao,Shuhuai Wang,Qingyuan Zhang

View all publications

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