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AB283505

Anti-NCR1/NKp46 antibody [mNCR1.05]

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(1 Publication)

Mouse Recombinant Monoclonal NCR1 antibody. Suitable for IHC-P, ELISA and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CD335, Ly94, Ncr1, Natural cytotoxicity triggering receptor 1, Activating receptor 1, Lymphocyte antigen 94, Natural killer cell p46-related protein, mAR-1, NK-p46, NKp46, mNKp46

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling NCR1 with ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in rat spleen.The section was incubated with ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling NCR1 with ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in mouse colon.The section was incubated with ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling NCR1 with ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in rat lung.The section was incubated with ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NCR1 with ab283505 at 100 (10.19 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in mouse spleen.The section was incubated with ab283505 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

ELISA - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)
  • ELISA

Lab

ELISA - Anti-NCR1/NKp46 antibody [mNCR1.05] (AB283505)

ELISA analysis using ab283505 at a range of 0-500 ng/ml followed by a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）at 1/1000 dilution. Antigen concentration : 1000 ng/ml.

Antigens : Mouse NKp46/NCR1 Fc Chimera Protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mNCR1.05

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat

Applications

ELISA, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.0625 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCR1 also known as NKp46 is a receptor mainly expressed on the surface of natural killer (NK) cells. Its primary role involves recognizing and binding to ligands present on target cells including tumor cells and virus-infected cells. NCR1 initiates the activation of NK cells leading to the destruction of these abnormal cells. The NCR1 protein has a molecular weight of approximately 46 kDa and is a part of the immunoglobulin superfamily. NCR1 is mainly expressed in the immune system specifically on NK cells which are an important component of the innate immune response.
Biological function summary

The function of NCR1 involves the recognition of stressed transformed or infected cells marking them for elimination by NK cells. It does not typically function as a part of a complex; rather it serves a somewhat autonomous role in cell signaling. However its interaction with other receptors on NK cells including DAP12 enhances its signaling capabilities. Through the activation of cascades NCR1 promotes the release of cytotoxic granules and the production of cytokines therefore mediating the immune response.

Pathways

NCR1 plays a significant role in the regulation of immune surveillance pathways. It is involved in the recognition and elimination pathways that modulate the immune response against infected or cancerous cells. NK cells with NCR1's involvement interact with receptors like NKG2D adjusting the NK cell's cytotoxic activity. These interactions coordinate the broader immune response important for innate immunity effectively bridging innate and adaptive immunity by influencing other immune cells.

NCR1 has been implicated in cancer and infectious diseases. In cancer altered expression or dysfunction of NCR1 may contribute to the evasion of immune surveillance by tumor cells potentially leading to tumor progression. Studies have shown reduced NKp46 expression in some cancerous states linking it to poor prognosis. In infectious diseases NCR1 plays a role in targeting virus-infected cells and its dysfunction can result in reduced effectiveness in clearing viral infections. Interactions with viral envelope proteins can interfere with NCR1 activity exemplifying its importance in antiviral defense mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
See full target information Ncr1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:1909 PubMed38429294

2024

synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle B Lupo,Xue Yao,Shambhavi Borde,Jiao Wang,Sandra Torregrosa-Allen,Bennett D Elzey,Sagar Utturkar,Nadia A Lanman,MacKenzie McIntosh,Sandro Matosevic
View all publications
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Product promise

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