AB14823

Anti-NCR1/NKp46 antibody [n1D9]

Name: Anti-NCR1/NKp46 antibody [n1D9] (ab14823) | Abcam
Brand: Abcam Limited
SKU: AB14823
Price: 500 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal NCR1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NCR1.

View Alternative Names

CD335, LY94, NCR1, Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46

2 Images

Flow Cytometry - Anti-NCR1/NKp46 antibody [n1D9] (AB14823)

Flow Cyt

Unknown

Flow Cytometry - Anti-NCR1/NKp46 antibody [n1D9] (AB14823)

Flow cytometry analysis of NKp46 in PBMC cells. The cell was stained with ab14823 at 2-5μg for 1x10⁶ cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Flow Cyt

Unknown

Flow Cytometry - Anti-NCR1/NKp46 antibody [n1D9] (AB14823)

ab14823 staining human PBMC by flow cytometry.

This image was produced using the ascites version of this antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

n1D9

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NCR1.

O76036

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCR1 also known as NKp46 is a receptor mainly expressed on the surface of natural killer (NK) cells. Its primary role involves recognizing and binding to ligands present on target cells including tumor cells and virus-infected cells. NCR1 initiates the activation of NK cells leading to the destruction of these abnormal cells. The NCR1 protein has a molecular weight of approximately 46 kDa and is a part of the immunoglobulin superfamily. NCR1 is mainly expressed in the immune system specifically on NK cells which are an important component of the innate immune response.

Biological function summary

The function of NCR1 involves the recognition of stressed transformed or infected cells marking them for elimination by NK cells. It does not typically function as a part of a complex; rather it serves a somewhat autonomous role in cell signaling. However its interaction with other receptors on NK cells including DAP12 enhances its signaling capabilities. Through the activation of cascades NCR1 promotes the release of cytotoxic granules and the production of cytokines therefore mediating the immune response.

Pathways

NCR1 plays a significant role in the regulation of immune surveillance pathways. It is involved in the recognition and elimination pathways that modulate the immune response against infected or cancerous cells. NK cells with NCR1's involvement interact with receptors like NKG2D adjusting the NK cell's cytotoxic activity. These interactions coordinate the broader immune response important for innate immunity effectively bridging innate and adaptive immunity by influencing other immune cells.

NCR1 has been implicated in cancer and infectious diseases. In cancer altered expression or dysfunction of NCR1 may contribute to the evasion of immune surveillance by tumor cells potentially leading to tumor progression. Studies have shown reduced NKp46 expression in some cancerous states linking it to poor prognosis. In infectious diseases NCR1 plays a role in targeting virus-infected cells and its dysfunction can result in reduced effectiveness in clearing viral infections. Interactions with viral envelope proteins can interfere with NCR1 activity exemplifying its importance in antiviral defense mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

See full target information NCR1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of cancer 143:419-429 PubMed29441566

2018

Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model.

Applications

Unspecified application

Species

Unspecified reactive species

Sifeng Qu,Hui Xue,Xin Dong,Dong Lin,Rebecca Wu,Noushin Nabavi,Colin C Collins,Martin E Gleave,Peter W Gout,Yuzhuo Wang

Cancer biology & therapy 17:1253-1265 PubMed27834580

2016

NKG2D ligand expression in pediatric brain tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen Haberthur,Kathryn Brennan,Virginia Hoglund,Stephanie Balcaitis,Harrison Chinn,Amira Davis,Shannon Kreuser,Conrad Winter,Sarah E S Leary,Gail H Deutsch,Richard G Ellenbogen,Courtney A Crane

Journal of translational medicine 11:145 PubMed23758773

2013

Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients.

Applications

IHC-P

Species

Unspecified reactive species

Maria Libera Ascierto,Michael O Idowu,Yingdong Zhao,Hanif Khalak,Kyle K Payne,Xiang-Yang Wang,Catherine I Dumur,Davide Bedognetti,Sara Tomei,Paolo A Ascierto,Anil Shanker,Harry D Bear,Ena Wang,Francesco M Marincola,Andrea De Maria,Masoud H Manjili

View all publications

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