AB177952

Anti-NCX1 antibody [EPR12739]

Name: Anti-NCX1 antibody [EPR12739] Rabbit monoclonal (ab177952) | Abcam
Brand: Abcam Limited
SKU: AB177952
Price: 590 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(24 Publications)

Rabbit Recombinant Monoclonal NCX1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 24 publications.

View Alternative Names

CNC, NCX1, SLC8A1, Sodium/calcium exchanger 1, Na(+)/Ca(2+)-exchange protein 1, Solute carrier family 8 member 1

2 Images

Lab

Western blot - Anti-NCX1 antibody [EPR12739] (AB177952)

This antibody detects high backgroud at high working concentration.

All lanes:

Western blot - Anti-NCX1 antibody [EPR12739] (ab177952)

All lanes:

Human heart lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 108 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-NCX1 antibody [EPR12739] (AB177952)

This antibody detects high backgroud at high working concentration.

All lanes:

Western blot - Anti-NCX1 antibody [EPR12739] (ab177952)

Lane 1:

Mouse heart tissue lysate at 15 µg

Lane 2:

Rat heart tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 108 kDa

Observed band size: 120 kDa

false

Carrier free

Anti-NCX1 antibody [EPR12739] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12739

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCX1 also known as the Sodium-Calcium Exchanger 1 plays a critical role in removing calcium ions from cells by exchanging them for sodium ions. It operates as an antiporter mechanism facilitating the transport of three sodium ions into the cell in exchange for one calcium ion being transported out. The protein has a molecular mass of approximately 120 kDa. NCX1 is widely expressed in various tissues including heart brain kidney and skeletal muscle indicating its importance in multiple physiological processes.

Biological function summary

NCX1 contributes significantly to controlling intracellular calcium levels important for muscle contraction and neuronal function. The protein forms part of a larger complex that includes several regulatory proteins which modulate its activity according to the cellular environment. By managing calcium ion concentration inside cells NCX1 influences various cellular processes such as muscle relaxation and excitatory signaling in neurons.

Pathways

NCX1 integrates itself into the cellular calcium homeostasis and cardiac excitation-contraction coupling pathways. In the cardiac tissue it works alongside other proteins like the L-type calcium channel contributing to the regulation of the heart’s rhythm and contraction force. Another pathway involves its interaction with the sodium-potassium ATPase which helps maintain the cell's electrochemical gradient essential for proper muscle and nerve function.

NCX1 has been linked to cardiovascular diseases such as heart failure and arrhythmias. Abnormal NCX1 function can disrupt calcium balance leading to these conditions. Moreover the protein's relationship with the L-type calcium channel is important because alterations in their interaction can exacerbate such cardiovascular disorders. Additionally NCX1 has connections to disorders like epilepsy where other ion channels and transporters also play critical roles in maintaining neural excitability and preventing seizure activities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed : 11241183, PubMed : 1374913, PubMed : 1476165). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed : 11241183, PubMed : 1374913, PubMed : 1476165). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum (PubMed : 11241183, PubMed : 1374913, PubMed : 1476165). SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed : 11241183, PubMed : 1374913, PubMed : 1476165). Required for normal embryonic heart development and the onset of heart contractions (By similarity).

See full target information SLC8A1

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed41007781

2025

Prior Aerobic Exercise Training Fails to Confer Cardioprotection Under Varying Exercise Volumes in Early Post-Infarction Cardiac Remodeling in Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

André Rodrigues Lourenço Dias,Ednei Luiz Antonio,Helenita Antonia de Oliveira,Ighor Luiz Azevedo Teixeira,Larissa Emília Seibt,Andrey Jorge Serra

Scientific reports 15:29469 PubMed40797070

2025

2-aminoethoxydiphenylborane intervenes intracellular calcium signaling and attenuates myocardial ischemia-reperfusion injury in mice through ITPR1/MCU pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hailong Bao,Xin Chen,Xi Hu,Zhaoxing Cao,Wei Zhou,Bingxiu Chen,Fujun Liao,Fang Wei,Runze Huang,Wei Li,Zhangrong Chen

Frontiers in pharmacology 16:1557685 PubMed40206075

2025

Allicin ameliorates acute myocardial infarction in rats by modulating calcium homeostasis in cardiomyocytes through the induction of hydrogen sulfide production.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyu Liu,Shaojun Xu,Juan Wang,Xinxia Li,Ruiting Liu,Le Zhao,Yikui Li,Rongmei Shi,Jinyan Zhang

Cell communication and signaling : CCS 22:258 PubMed38711131

2024

NCX1/Ca promotes autophagy and decreases bortezomib activity in multiple myeloma through non-canonical NFκB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Li,Pingping Xiao,Dongbiao Qiu,Apeng Yang,Qingjiao Chen,Junfang Lin,Yao Liu,Junmin Chen,Zhiyong Zeng

Nature communications 14:3346 PubMed37291092

2023

SERCA2 phosphorylation at serine 663 is a key regulator of Ca homeostasis in heart diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Fabrice Gonnot,Laura Boulogne,Camille Brun,Maya Dia,Yves Gouriou,Gabriel Bidaux,Christophe Chouabe,Claire Crola Da Silva,Sylvie Ducreux,Bruno Pillot,Andrea Kaczmarczyk,Christelle Leon,Stephanie Chanon,Coralie Perret,Franck Sciandra,Tanushri Dargar,Vincent Gache,Fadi Farhat,Laurent Sebbag,Thomas Bochaton,Helene Thibault,Michel Ovize,Melanie Paillard,Ludovic Gomez

Redox biology 59:102594 PubMed36603528

2023

Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jikai Zhao,Liming Yu,Xiaodong Xue,Yinli Xu,Tao Huang,Dengyue Xu,Zhishang Wang,Linyu Luo,Huishan Wang

Clinical and experimental medicine 23:1581-1596 PubMed36251145

2022

NCX1 disturbs calcium homeostasis and promotes RANKL-induced osteoclast differentiation by regulating JNK/c-Fos/NFATc1 signaling pathway in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Li,Dongbiao Qiu,Qingjiao Chen,Apeng Yang,Junmin Chen,Zhiyong Zeng

Biomedicines 10: PubMed35884952

2022

β-Adrenergic Receptor Stimulation Maintains NCX-CaMKII Axis and Prevents Overactivation of -Signaling in Cardiomyocytes upon Increased Workload.

Applications

Unspecified application

Species

Unspecified reactive species

Ingrid Matzer,Julia Voglhuber,Mara Kiessling,Nataša Djalinac,Viktoria Trummer-Herbst,Nishani Mabotuwana,Lavinia Rech,Michael Holzer,Samuel Sossalla,Peter P Rainer,Andreas Zirlik,Senka Ljubojevic-Holzer

Cells 11: PubMed35456008

2022

Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Asima Tayyeb,Gry H Dihazi,Björn Tampe,Michael Zeisberg,Desiree Tampe,Samy Hakroush,Charlotte Bührig,Jenny Frese,Nazli Serin,Marwa Eltoweissy,Gerhard A Müller,Hassan Dihazi

International journal of molecular sciences 23: PubMed35055084

2022

Suppressing Effect of Na/Ca Exchanger (NCX) Inhibitors on the Growth of Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zikai Liu,Qing Cheng,Xiaoli Ma,Mingke Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCX1 antibody [EPR12739]

Western blot - Anti-NCX1 antibody [EPR12739] (AB177952)

Western blot - Anti-NCX1 antibody [EPR12739] (AB177952)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

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