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AB214224

Anti-ND6 antibody

0

(1 Review)

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(2 Publications)

Rabbit Polyclonal ND6 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MT-ND6 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MTND6, NADH6, ND6, MT-ND6, NADH-ubiquinone oxidoreductase chain 6, NADH dehydrogenase subunit 6

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ND6 antibody (AB214224)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ND6 antibody (AB214224)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling ND6 with ab214224 at 1/100 dilution, followed by secondary antibody detection and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ND6 antibody (AB214224)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ND6 antibody (AB214224)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ND6 with ab214224 at 1/400 dilution, followed by secondary antibody detection and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MT-ND6 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P03923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ND6 also known as NADH-ubiquinone oxidoreductase chain 6 is part of the mitochondrial respiratory chain complex I. This protein contributes to the electron transport chain and helps catalyze the transfer of electrons from NADH to ubiquinone. ND6 has a mass of about 17 kDa and resides in the inner mitochondrial membrane. It is expressed in various tissues with high energy demand such as muscle heart and brain reflecting its role in oxidative phosphorylation.
Biological function summary

ND6 contributes to cellular energy production. ND6 is an integral component of complex I which consists of 45 different subunits. This complex is the first and largest enzyme of the mitochondrial electron transport chain and plays a significant role in ATP production. By participating in the proton gradient creation across the mitochondrial membrane ND6 aids in maintaining proper energy levels in cells.

Pathways

ND6 functions within important pathways like oxidative phosphorylation and cellular respiration. It works closely with subunits such as ND1 ND2 and others in complex I to facilitate electron flow and ATP synthesis. The electron transport chain requires the coordinated activity of these proteins to achieve maximum efficiency in energy conversion from metabolic substrates.

ND6 mutations have been linked to conditions such as Leber's hereditary optic neuropathy and mitochondrial encephalomyopathy. ND6 alterations can impair energy production leading to cellular dysfunction typical in these disorders. Proteins like ND4 also part of complex I often exhibit synergistic mutations contributing to the severity of associated mitochondrial diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 14595656, PubMed : 8644732). Essential for the catalytic activity and assembly of complex I (PubMed : 14595656, PubMed : 8644732).
See full target information MT-ND6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed37893184

2023

DLL3 Is a Prognostic and Potentially Predictive Biomarker for Immunotherapy Linked to PD/PD-L Axis and NOTCH1 in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Lacalle-Gonzalez,Maria Florez-Cespedes,Lara Sanz-Criado,Michael Ochieng' Otieno,Edurne Ramos-Muñoz,Maria Jesus Fernandez-Aceñero,Luis Ortega-Medina,Jesus Garcia-Foncillas,Javier Martinez-Useros

Neurobiology of disease 159:105483 PubMed34400304

2021

Neuroglobin effectively halts vision loss in Harlequin mice at an advanced stage of optic nerve degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hélène Cwerman-Thibault,Christophe Lechauve,Vassilissa Malko-Baverel,Sébastien Augustin,Gwendoline Le Guilloux,Élodie Reboussin,Julie Degardin-Chicaud,Manuel Simonutti,Thomas Debeir,Marisol Corral-Debrinski
View all publications
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