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Rabbit Recombinant Monoclonal NDE1 antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-NDE1 antibody [EPR30443-553] (AB324144), expandable thumbnail
  • Western blot - Anti-NDE1 antibody [EPR30443-553] (AB324144), expandable thumbnail
  • Western blot - Anti-NDE1 antibody [EPR30443-553] (AB324144), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.

Alternative names

NUDE, Nuclear distribution protein nudE homolog 1, NudE

Rabbit Recombinant Monoclonal NDE1 antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR30443-553
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144), expandable thumbnail

    Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144)

    NDE1 Western blot staining using rabbit Anti-NDE1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: spleen

    Lane 3-4 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

    Lane 2: HeLa transfected with siRNA specifically targeting NDE1 whole cell lysate at 40 µg

    Lane 3: Human brainstem tissue lysate at 40 µg

    Lane 4: Human spleen tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144), expandable thumbnail

    Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144)

    NDE1 Western blot staining using rabbit Anti-NDE1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HepG2, IMR-90

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144) at 1/1000 dilution

    Lane 1: Mouse cerebral cortex tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 7: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 8: IMR-90 (human lung fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 125s

  • Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144), expandable thumbnail

    Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144)

    NDE1 Western blot staining using rabbit Anti-NDE1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: heart

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 125s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com