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Rabbit Recombinant Multiclonal NDE1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NDE1.

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Images

Western blot - Anti-NDE1 Antibody [RP23040189] (AB313415), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human NDE1. Database link Q9NXR1

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Target data

Function

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.

Alternative names

NUDE, NDE1, Nuclear distribution protein nudE homolog 1, NudE

Recommended products

Rabbit Recombinant Multiclonal NDE1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NDE1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human NDE1. Database link Q9NXR1
Clone number
RP23040189
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nucleocyte dynein axonemal 1 also known as NDE1 is an important protein involved in cell division mechanics. It weighs approximately 33 kDa. NDE1 is expressed strongly in the brain particularly in neural progenitor cells and helps in maintaining centrosome function and microtubule organization. Due to its role in centrosome attachment NDE1 plays an important role in chromosomal alignment and segregation during cell division.

Biological function summary

NDE1 participates in centrosomal and microtubule functions. It forms a complex with other proteins such as LIS1 and dynein important for spindle positioning in dividing cells. The NDE1 complex ensures the correct orientation and symmetrical division of proliferating cells impacting tissue architecture and brain development. By partnering with these proteins NDE1 helps maintain the structural integrity of mitotic spindles.

Pathways

NDE1 contributes significantly to the cell cycle and neurogenesis pathways. It interacts with the dynein motor complex facilitating dynein-mediated transport and positioning in dividing neurons. Within these pathways proteins such as LIS1 and DISC1 work with NDE1 indicating its broad impact on cellular positioning and neural development. NDE1 helps regulate the pathways which ensure precise cell division and brain formation.

Associated diseases and disorders

NDE1 has links to microcephaly and lissencephaly. Mutations in NDE1 contribute to microcephaly by disrupting neuronal proliferation and migration. The protein interacts with LIS1 in this disorder which shares roles in neurodevelopment. Similarly lissencephaly characterized by smooth brain structures due to abnormal neuronal migration shows association with NDE1 dysfunction. Therefore NDE1 is significant for both normal brain development and related disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Western blot - Anti-NDE1 Antibody [RP23040189] (ab313415), expandable thumbnail

    Western blot - Anti-NDE1 Antibody [RP23040189] (ab313415)

    All lanes: Western blot - Anti-NDE1 Antibody [RP23040189] (ab313415) at 1 µg/mL

    Lane 1: Non-transfected HeLa cell lysate

    Lane 2: Non-specific scrambled siRNA transfected HeLa cell lysate

    Lane 3: NDE1 knock down HeLa cell lysate

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Observed band size: 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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