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AB124895

Anti-NDEL1 antibody [EPR5068]

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(3 Publications)

Rabbit Recombinant Monoclonal NDEL1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

EOPA, MITAP1, NUDEL, NDEL1, Nuclear distribution protein nudE-like 1, Protein Nudel, Mitosin-associated protein 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDEL1 antibody [EPR5068] (AB124895)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDEL1 antibody [EPR5068] (AB124895)

ab124895 at 1/50 dilution staining NDEL1 in Formalin-fixed Paraffin-embedded Human Brain tissue by Immunohistochemistry

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-NDEL1 antibody [EPR5068] (AB124895)
  • WB

Unknown

Western blot - Anti-NDEL1 antibody [EPR5068] (AB124895)

All lanes:

Western blot - Anti-NDEL1 antibody [EPR5068] (ab124895) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 38 kDa

false

OI-RD Scanning - Anti-NDEL1 antibody [EPR5068] (AB124895)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NDEL1 antibody [EPR5068] (AB124895)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-NDEL1 antibody [EPR5068] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5068

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDEL1 also known as Nudel or nuclear distribution element-like 1 is a protein with a molecular mass of around 38 kDa. It plays a role mechanically by participating in microtubule and centrosome-related processes. In terms of expression NDEL1 is found in both the central nervous system and several peripheral tissues. Its localization relates to its function in cellular dynamics particularly involving the cytoskeleton.
Biological function summary

NDEL1 interacts within a multi-protein complex called the dynein motor complex. This association is critical for the process of neuronal migration and axonal transport involving the movement of cellular components along microtubules. The protein acts as a linking agent facilitating microtubule assembly and organization which are essential for maintaining cellular structure and function.

Pathways

The role of NDEL1 in cytoskeletal dynamics integrates into key pathways such as the cell cycle and neurodevelopmental processes. It interacts with proteins like LIS1 in the cell cycle pathway which affects mitosis. Moreover its interaction with dynein emphasizes its role in intracellular transport during neurodevelopment indicating its aosphysical involvement in brain architecture.

NDEL1 has a significant connection with neurodevelopmental disorders and neurodegenerative diseases. Its association with LIS1 and the disruption of their interaction contributes to lissencephaly a condition marked by smooth brain surface. Additionally alterations in NDEL1 function also connect with Alzheimer's disease due to its role in neuronal structural maintenance alongside proteins such as tau that become dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).
See full target information NDEL1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37175528

2023

A Novel Rabbit Anti-Myoglobin Monoclonal Antibody's Potential Application in Rhabdomyolysis Associated Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Wang,Ou Qiao,Lu Han,Ning Li,Yanhua Gong

Scientific reports 12:16493 PubMed36192543

2022

Activation of RhoC by regulatory ubiquitination is mediated by LNX1 and suppressed by LIS1.

Applications

Unspecified application

Species

Unspecified reactive species

Stanislav Kholmanskikh,Shawn Singh,M Elizabeth Ross

Neural regeneration research 17:401-408 PubMed34269216

2021

miR-103-3p targets Ndel1 to regulate neural stem cell proliferation and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Li,Shan-Shan Wang,Bo-Quan Shan,Jian-Bing Qin,He-Yan Zhao,Mei-Ling Tian,Hui He,Xiang Cheng,Xin-Hua Zhang,Guo-Hua Jin
View all publications
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