Skip to main content

Rabbit Recombinant Monoclonal NDEL1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (AB248046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (AB248046), expandable thumbnail
  • OI-RD Scanning - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (AB248046), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Predicted
Not recommended
Not recommended
Predicted
Not recommended
Rat
Predicted
Not recommended
Not recommended
Predicted
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).

    Alternative names

    Nuclear distribution protein nudE-like 1, Protein Nudel, Mitosin-associated protein 1, EOPA, NUDEL, MITAP1, NDEL1

    Recommended products

    1. Loading...
    2. Loading...
    3. Loading...
    4. Loading...

    Rabbit Recombinant Monoclonal NDEL1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

    Alternative names

    Nuclear distribution protein nudE-like 1, Protein Nudel, Mitosin-associated protein 1, EOPA, NUDEL, MITAP1, NDEL1

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR5068

    Purification technique

    Affinity purification Protein A

    Dissociation constant

    2.9 x 10-12 M

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Storage information

    Do Not Freeze

    Notes

    ab248046 is the carrier-free version of Anti-NDEL1 antibody [EPR5068] ab124895.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    NDEL1 also known as Nudel or nuclear distribution element-like 1 is a protein with a molecular mass of around 38 kDa. It plays a role mechanically by participating in microtubule and centrosome-related processes. In terms of expression NDEL1 is found in both the central nervous system and several peripheral tissues. Its localization relates to its function in cellular dynamics particularly involving the cytoskeleton.

    Biological function summary

    NDEL1 interacts within a multi-protein complex called the dynein motor complex. This association is critical for the process of neuronal migration and axonal transport involving the movement of cellular components along microtubules. The protein acts as a linking agent facilitating microtubule assembly and organization which are essential for maintaining cellular structure and function.

    Pathways

    The role of NDEL1 in cytoskeletal dynamics integrates into key pathways such as the cell cycle and neurodevelopmental processes. It interacts with proteins like LIS1 in the cell cycle pathway which affects mitosis. Moreover its interaction with dynein emphasizes its role in intracellular transport during neurodevelopment indicating its aosphysical involvement in brain architecture.

    Associated diseases and disorders

    NDEL1 has a significant connection with neurodevelopmental disorders and neurodegenerative diseases. Its association with LIS1 and the disruption of their interaction contributes to lissencephaly a condition marked by smooth brain surface. Additionally alterations in NDEL1 function also connect with Alzheimer's disease due to its role in neuronal structural maintenance alongside proteins such as tau that become dysregulated.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    3 product images

    • Western blot - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (ab248046), expandable thumbnail

      Western blot - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (ab248046)

      This data was developed using Anti-NDEL1 antibody [EPR5068] ab124895, the same antibody clone in a different buffer formulation.

      All lanes: Western blot - Anti-NDEL1 antibody [EPR5068] (Anti-NDEL1 antibody [EPR5068] ab124895) at 1/1000 dilution

      Lane 1: SH-SY5Y cell lysate at 10 µg

      Lane 2: HeLa cell lysate at 10 µg

      Lane 3: Jurkat lysate at 10 µg

      Lane 4: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

      Predicted band size: 38 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (ab248046), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (ab248046)

      This data was developed using Anti-NDEL1 antibody [EPR5068] ab124895, the same antibody clone in a different buffer formulation.Anti-NDEL1 antibody [EPR5068] ab124895, at 1/50 dilution, staining NDEL1 in Formalin-fixed, Paraffin-embedded Human Brain tissue by Immunohistochemistry

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (ab248046), expandable thumbnail

      OI-RD Scanning - Anti-NDEL1 antibody [EPR5068] - BSA and Azide free (ab248046)

      We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
      Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com