Rabbit Polyclonal NDNL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NSMCE3.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human NSMCE3. The exact immunogen used to generate this antibody is proprietary information. Database link Q96MG7
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination (PubMed:20864041, PubMed:27427983). The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (PubMed:20864041). May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest (By similarity).
Alternative names
HCA4, MAGEG1, NDNL2, NSMCE3, Non-structural maintenance of chromosomes element 3 homolog, Non-SMC element 3 homolog, Hepatocellular carcinoma-associated protein 4, MAGE-G1 antigen, Melanoma-associated antigen G1, Necdin-like protein 2
Recommended products
Rabbit Polyclonal NDNL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NSMCE3.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human NSMCE3. The exact immunogen used to generate this antibody is proprietary information. Database link Q96MG7
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NDNL2 protein known as Necdin-Like 2 operates mechanically as a membrane-associated protein involved in signaling pathways. It has an approximate mass of 55 kDa. Scientists mostly find NDNL2 expressed in the brain but there is expression in other tissues as well. This protein participates in intracellular interactions and helps maintain cellular communication.
- Biological function summary
Its role centers around regulatory processes that affect cellular proliferation and differentiation. NDNL2 acts as part of a complex that influences neuronal development showing its importance in the nervous system. It interacts closely with other proteins involved in neurogenesis and cellular morphogenesis facilitating structured neuronal growth.
- Pathways
NDNL2 takes part in signaling pathways like the PI3K/AKT and MAPK pathways. These pathways include critical involvement of proteins such as Akt and ERK which are key in processes like cell survival growth and apoptosis. NDNL2 contributes to modulating these pathways therefore impacting cell function and morphology.
- Associated diseases and disorders
NDNL2 shows connections to neurodevelopmental disorders and certain types of cancers. Its interaction with proteins like p53 and RTK in cancer cases points to its role in maintaining cellular integrity and preventing malignancy. The dysfunction or altered expression of NDNL2 can lead to neurodevelopmental issues highlighting its influence on healthy brain function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-NDNL2 antibody (ab198686)
10% SDS-Page
All lanes: Western blot - Anti-NDNL2 antibody (ab198686) at 1/1500 dilution
Lane 1: Mouse liver tissue lysate at 40 µg
Lane 2: A172 cell lysate at 40 µg
Lane 3: Human prostate lysate at 40 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDNL2 antibody (ab198686)
Immunohistochemical analysis of paraffin embedded Human gastric cancer tissue labeling NDNL2 with ab198686 at 1/40 dilution.
Downloads
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