AB198686

Anti-NDNL2 antibody

Name: Anti-NDNL2 antibody (ab198686) | Abcam
Brand: Abcam Limited
SKU: AB198686

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(1 Publication)

Rabbit Polyclonal NDNL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NSMCE3.

View Alternative Names

HCA4, MAGEG1, NDNL2, NSMCE3, Non-structural maintenance of chromosomes element 3 homolog, Non-SMC element 3 homolog, Hepatocellular carcinoma-associated protein 4, MAGE-G1 antigen, Melanoma-associated antigen G1, Necdin-like protein 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDNL2 antibody (AB198686)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDNL2 antibody (AB198686)

Immunohistochemical analysis of paraffin embedded Human gastric cancer tissue labeling NDNL2 with ab198686 at 1/40 dilution.

Supplier Data

Western blot - Anti-NDNL2 antibody (AB198686)

10% SDS-Page

All lanes:

Western blot - Anti-NDNL2 antibody (ab198686) at 1/1500 dilution

Lane 1:

Mouse liver tissue lysate at 40 µg

Lane 2:

A172 cell lysate at 40 µg

Lane 3:

Human prostate lysate at 40 µg

Predicted band size: 34 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NSMCE3. The exact immunogen used to generate this antibody is proprietary information.

Q96MG7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NDNL2 protein known as Necdin-Like 2 operates mechanically as a membrane-associated protein involved in signaling pathways. It has an approximate mass of 55 kDa. Scientists mostly find NDNL2 expressed in the brain but there is expression in other tissues as well. This protein participates in intracellular interactions and helps maintain cellular communication.

Biological function summary

Its role centers around regulatory processes that affect cellular proliferation and differentiation. NDNL2 acts as part of a complex that influences neuronal development showing its importance in the nervous system. It interacts closely with other proteins involved in neurogenesis and cellular morphogenesis facilitating structured neuronal growth.

Pathways

NDNL2 takes part in signaling pathways like the PI3K/AKT and MAPK pathways. These pathways include critical involvement of proteins such as Akt and ERK which are key in processes like cell survival growth and apoptosis. NDNL2 contributes to modulating these pathways therefore impacting cell function and morphology.

NDNL2 shows connections to neurodevelopmental disorders and certain types of cancers. Its interaction with proteins like p53 and RTK in cancer cases points to its role in maintaining cellular integrity and preventing malignancy. The dysfunction or altered expression of NDNL2 can lead to neurodevelopmental issues highlighting its influence on healthy brain function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination (PubMed : 20864041, PubMed : 27427983). The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3 : substrate complex (PubMed : 20864041). May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest (By similarity).

See full target information NSMCE3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 9:e10651 PubMed39553435

2024

Siglec15/TGF-β bispecific antibody mediates synergistic anti-tumor response against 4T1 triple negative breast cancer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Limei Shen,Alison M Schaefer,Karthik Tiruthani,Whitney Wolf,Samuel K Lai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDNL2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDNL2 antibody (AB198686)

Western blot - Anti-NDNL2 antibody (AB198686)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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