Anti-NDP52 antibody [OTI4H5]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-NDP52 antibody [OTI4H5] (AB124372)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling NDP52 using ab124372 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NDP52 antibody [OTI4H5] (AB124372)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either NDP52 overexpress plasmid (Red) or empty vector control plasmid (Blue) labeling NDP52 with ab124372 at 1/100 dilution.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NDP52 antibody [OTI4H5] (AB124372)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling NDP52 using ab124372 at 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).

• WB

Lab

Western blot - Anti-NDP52 antibody [OTI4H5] (AB124372)

Western blot : Anti-CALCOCO2 antibody [OTI4H5] (ab124372) staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (ab108198) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab124372 was shown to bind specifically to CALCOCO2. A band was observed at 57 kDa in wild-type A549 cell lysates with no signal observed at this size in CALCOCO2 knockout cell line. To generate this image, wild-type and CALCOCO2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-NDP52 antibody [OTI4H5] (ab124372) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

CALCOCO2 knockout A549 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 57 kDa

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• WB

Supplier Data

Western blot - Anti-NDP52 antibody [OTI4H5] (AB124372)

All lanes:

Western blot - Anti-NDP52 antibody [OTI4H5] (ab124372) at 1/500 dilution

Lane 1:

Human testis extract at 10 µg

Lane 2:

Human omentum extract at 10 µg

Lane 3:

Human uterus extract at 10 µg

Lane 4:

Human breast extract at 10 µg

Lane 5:

Human brain extract at 10 µg

Lane 6:

Human liver extract at 10 µg

Lane 7:

Human ovary extract at 10 µg

Lane 8:

Human thyroid gland extract at 10 µg

Lane 9:

Human colon extract at 10 µg

Predicted band size: 52 kDa

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• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NDP52 antibody [OTI4H5] (AB124372)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY NDP52, labeling NDP52 using ab124372 at dilution 1/100 dilution in ICC/IF.

• WB

Supplier Data

Western blot - Anti-NDP52 antibody [OTI4H5] (AB124372)

All lanes:

Western blot - Anti-NDP52 antibody [OTI4H5] (ab124372) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 52 kDa

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• WB

Unknown

Western blot - Anti-NDP52 antibody [OTI4H5] (AB124372)

All lanes:

Western blot - Anti-NDP52 antibody [OTI4H5] (ab124372) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY NDP52 cDNA at 5 µg

Predicted band size: 52 kDa

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