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AB247976

Anti-NDRG1 (phospho S330) antibody [EPR5594] - BSA and Azide free

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Rabbit Recombinant Monoclonal NDRG1 phospho S330 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CAP43, DRG1, RTP, NDRG1, Protein NDRG1, Differentiation-related gene 1 protein, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, Rit42, DRG-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 (phospho S330) antibody [EPR5594] - BSA and Azide free (AB247976)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 (phospho S330) antibody [EPR5594] - BSA and Azide free (AB247976)

This data was developed using ab124713, the same antibody clone in a different buffer formulation.

ab124713, at 1/100, staining NDRG1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-NDRG1 (phospho S330) antibody [EPR5594] - BSA and Azide free (AB247976)
  • WB

Unknown

Western blot - Anti-NDRG1 (phospho S330) antibody [EPR5594] - BSA and Azide free (AB247976)

This data was developed using ab124713, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDRG1 (phospho S330) antibody [EPR5594] (<a href='/en-us/products/primary-antibodies/ndrg1-phospho-s330-antibody-epr5594-ab124713'>ab124713</a>) at 1/10000 dilution

Lane 1:

Untreated 293T cell lysate at 10 µg

Lane 2:

Calyculin A treated 293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 43 kDa

false

OI-RD Scanning - Anti-NDRG1 (phospho S330) antibody [EPR5594] - BSA and Azide free (AB247976)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NDRG1 (phospho S330) antibody [EPR5594] - BSA and Azide free (AB247976)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5594

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab247976 is the carrier-free version of ab124713.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDRG1 or N-Myc downstream-regulated gene 1 also known as Cap43 Drg-1 and RTP is a protein that plays key roles in various cellular processes. It has a molecular mass of approximately 43 kDa. NDRG1 is ubiquitously expressed with higher levels in the brain heart liver and kidney. The protein also exhibits a significant presence in cytoplasm but can translocate to the nucleus depending on the cellular context and stimuli.
Biological function summary

NDRG1 functions in cell growth regulation stress response and apoptosis influencing differentiation and potentially acting as a tumor suppressor. It does not directly form part of a larger protein complex but interacts with other proteins to mediate its effects. Its involvement in cellular homeostasis places it as a significant player in maintaining normal cell physiology.

Pathways

NDRG1 is involved in the hypoxia and stress response pathways regulating cellular responses to low oxygen and oxidative stress. It interacts with HIF-1α a critical regulator of the cellular response to hypoxia and also modulates PTEN and p53 proteins that are important for cell cycle control and apoptosis. These pathways and interactions reflect its function in cellular regulatory networks.

NDRG1 has associations with cancer and Charcot-Marie-Tooth disease type 4D. In cancer abnormal expression of NDRG1 has been linked to tumor progression and metastasis where it interacts with proteins like E-cadherin to influence cell adhesion and migration. In Charcot-Marie-Tooth disease mutations in the NDRG1 gene disrupt normal nerve function pointing to its importance in peripheral nervous system health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.
See full target information NDRG1 phospho S330
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com