AB307707

Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Knockout Tested Rabbit Recombinant Monoclonal NDRG1 phospho T346 antibody. Carrier free. Suitable for WB, IP, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.

View Alternative Names

CAP43, DRG1, RTP, NDRG1, Protein NDRG1, Differentiation-related gene 1 protein, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, Rit42, DRG-1

6 Images

Supplier Data

Immunoprecipitation - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

This data was developed using ab307706, the same antibody clone in a different buffer formulation. NDRG1 (phospho T346) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) treated with 100nM Calyculin A for 30 minutes, whole cell lysate with ab307706 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307706 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : 293T treated with 100nM Calyculin A for 30 minutes, whole cell lysate Lane 2 : ab307706 IP in 293T treated with 100nM Calyculin A for 30 minutes, whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307706 in 293T treated with 100nM Calyculin A for 30 minutes, whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] (<a href='/en-us/products/primary-antibodies/ndrg1-phospho-t346-antibody-epr26325-247-ab307706'>ab307706</a>) at 1/30 dilution

All lanes:

293T treated with 100nM Calyculin A, whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 48 kDa

false

Exposure time: 5s

Supplier Data

Immunoprecipitation - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

This data was developed using ab307706, the same antibody clone in a different buffer formulation. NDRG1 (phospho T346) was immunoprecipitated from 0.35 mg C2C12 (mouse myoblast) treated with 20nM insulin A for 30 minutes, whole cell lysate with ab307706 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307706 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : C2C12 treated with 20nM insulin A for 30 minutes, whole cell lysate Lane 2 : ab307706 IP in C2C12 treated with 20nM insulin A for 30 minutes, whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307706 in C2C12 treated with 20nM insulin A for 30 minutes, whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] (<a href='/en-us/products/primary-antibodies/ndrg1-phospho-t346-antibody-epr26325-247-ab307706'>ab307706</a>) at 1/30 dilution

All lanes:

C2C12 treated with 20nM insulin A, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 48 kDa

false

Exposure time: 32s

Supplier Data

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

This data was developed using ab307706, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. LY294002 treatment decreased the expression of phospho T346 NDRG1(PMID : 18787837). In Western blot, anti-NDRG1 antibody (ab124689) Pan control staining at 1/1000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Alkaline phosphatase was used to treated membrane. Exposure time : 103 seconds.

All lanes:

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] (<a href='/en-us/products/primary-antibodies/ndrg1-phospho-t346-antibody-epr26325-247-ab307706'>ab307706</a>) at 1/1000 dilution

Lane 1:

HeLa starved for 4 hours, whole cell lysate at 20 µg

Lane 2:

HeLa starved for 4 hours, then treated with 50uM LY294002 for 4 hours, whole cell lysate at 20 µg

Lane 3:

HeLa starved for 4 hours, whole cell lysate (phosphatase treated membrane)

Lane 4:

HeLa starved for 4 hours, then treated with 50uM LY294002 for 4 hours, whole cell lysate (phosphatase treated membrane)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Observed band size: 48 kDa

false

Exposure time: 103s

Supplier Data

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

This data was developed using ab307706, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, anti-NDRG1 antibody (ab124689) Pan control staining at 1/1000 dilution.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] (<a href='/en-us/products/primary-antibodies/ndrg1-phospho-t346-antibody-epr26325-247-ab307706'>ab307706</a>) at 1/1000 dilution

Lane 1:

Wild-type 293T whole cell lysate at 20 µg

Lane 2:

293T treated with 100 nM Calyculin A, whole cell lysate at 20 µg

Lane 3:

NDRG1 knockout 293T whole cell lysate

Lane 4:

NDRG1 knockout 293T treated with 100 nM Calyculin A for 30 minutes whole cell lysate

Lane 5:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 43 kDa

Observed band size: 48 kDa

true

Exposure time: 59s

Supplier Data

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

This data was developed using ab307706, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Insulin treatment increased the expression of phospho T346 NDRG1 (PMID : 18787837). In Western blot, anti-NDRG1 antibody (ab124689) Pan control staining at 1/1000 dilution. In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : Lane 1 and 2 : 70 seconds, Lane 3 and 4 : 48 seconds.

All lanes:

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] (<a href='/en-us/products/primary-antibodies/ndrg1-phospho-t346-antibody-epr26325-247-ab307706'>ab307706</a>) at 1/1000 dilution

Lane 1:

C2C12 whole cell lysate at 20 µg

Lane 2:

C2C12 treated with 20nM insulin, whole cell lysate at 20 µg

Lane 3:

Rat-1 starved for 4 hours, whole cell lysate at 20 µg

Lane 4:

Rat-1 starved for 4 hours, then treated with 20nM insulin, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Observed band size: 48 kDa

false

Exposure time: 70s

Supplier Data

Dot Blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

This data was developed using ab307706, the same antibody clone in a different buffer formulation. Dot blot analysis of NDRG1 (phospho T346) using ab307706 at 1 : 1000 (0.513 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lane 1 : NDRG1 (phospho T346) peptide a Lane 2 : NDRG1 (phospho T346) peptide b Lane 3 : NDRG1 (phospho T350) peptide c Lane 4 : NDRG1 non-phospho peptide

Unconjugated

Anti-NDRG1 (phospho T346) antibody [EPR26325-247]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26325-247

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDRG1 or N-Myc downstream-regulated gene 1 also known as Cap43 Drg-1 and RTP is a protein that plays key roles in various cellular processes. It has a molecular mass of approximately 43 kDa. NDRG1 is ubiquitously expressed with higher levels in the brain heart liver and kidney. The protein also exhibits a significant presence in cytoplasm but can translocate to the nucleus depending on the cellular context and stimuli.

Biological function summary

NDRG1 functions in cell growth regulation stress response and apoptosis influencing differentiation and potentially acting as a tumor suppressor. It does not directly form part of a larger protein complex but interacts with other proteins to mediate its effects. Its involvement in cellular homeostasis places it as a significant player in maintaining normal cell physiology.

Pathways

NDRG1 is involved in the hypoxia and stress response pathways regulating cellular responses to low oxygen and oxidative stress. It interacts with HIF-1α a critical regulator of the cellular response to hypoxia and also modulates PTEN and p53 proteins that are important for cell cycle control and apoptosis. These pathways and interactions reflect its function in cellular regulatory networks.

NDRG1 has associations with cancer and Charcot-Marie-Tooth disease type 4D. In cancer abnormal expression of NDRG1 has been linked to tumor progression and metastasis where it interacts with proteins like E-cadherin to influence cell adhesion and migration. In Charcot-Marie-Tooth disease mutations in the NDRG1 gene disrupt normal nerve function pointing to its importance in peripheral nervous system health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.

See full target information NDRG1 phospho T346

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free

Immunoprecipitation - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

Immunoprecipitation - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

Western blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

Dot Blot - Anti-NDRG1 (phospho T346) antibody [EPR26325-247] - BSA and Azide free (AB307707)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise