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AB278702

Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5]

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(1 Publication)

Rabbit Recombinant Monoclonal NDRG1 phospho T346 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NDRG1 phospho T346.

View Alternative Names

CAP43, DRG1, RTP, NDRG1, Protein NDRG1, Differentiation-related gene 1 protein, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, Rit42, DRG-1

3 Images
Flow Cytometry - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (AB278702)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (AB278702)

Peptide blocking flow cytometric analysis of THP1 cells secondary antibody only negative control (light blue) or untreated (orange) using isotype control antibody #2141 or untreated (red) or with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278702 at 0.05 µg/mL.

Flow Cytometry - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (AB278702)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (AB278702)

Flow cytometric analysis of THP1 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using ab278702 at 0.05 µg/mL.

Western blot - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (AB278702)
  • WB

Supplier Data

Western blot - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (AB278702)

All lanes:

Western blot - Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5] (ab278702) at 0.05 µg/mL

Lane 1:

C2C12 (mouse myoblast cell line) whole cell lysate, untreated

Lane 2:

C2C12 (mouse myoblast cell line) whole cell lysate, treated with LY294002

Predicted band size: 43 kDa

false

  • 578 PE

    PE Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5]

  • 519 FITC

    FITC Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5]

  • 660 APC

    APC Anti-NDRG1 (phospho T346) antibody [NDRG1T346-F5]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

NDRG1T346-F5

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human NDRG1 phospho T346. The exact immunogen used to generate this antibody is proprietary information.

Q92597

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDRG1 or N-Myc downstream-regulated gene 1 also known as Cap43 Drg-1 and RTP is a protein that plays key roles in various cellular processes. It has a molecular mass of approximately 43 kDa. NDRG1 is ubiquitously expressed with higher levels in the brain heart liver and kidney. The protein also exhibits a significant presence in cytoplasm but can translocate to the nucleus depending on the cellular context and stimuli.
Biological function summary

NDRG1 functions in cell growth regulation stress response and apoptosis influencing differentiation and potentially acting as a tumor suppressor. It does not directly form part of a larger protein complex but interacts with other proteins to mediate its effects. Its involvement in cellular homeostasis places it as a significant player in maintaining normal cell physiology.

Pathways

NDRG1 is involved in the hypoxia and stress response pathways regulating cellular responses to low oxygen and oxidative stress. It interacts with HIF-1α a critical regulator of the cellular response to hypoxia and also modulates PTEN and p53 proteins that are important for cell cycle control and apoptosis. These pathways and interactions reflect its function in cellular regulatory networks.

NDRG1 has associations with cancer and Charcot-Marie-Tooth disease type 4D. In cancer abnormal expression of NDRG1 has been linked to tumor progression and metastasis where it interacts with proteins like E-cadherin to influence cell adhesion and migration. In Charcot-Marie-Tooth disease mutations in the NDRG1 gene disrupt normal nerve function pointing to its importance in peripheral nervous system health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.
See full target information NDRG1 phospho T346
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuropsychiatric disease and treatment 21:1911-1933 PubMed40926795

2025

Integrated Gut Microbiota, Metabolomics, and Network Pharmacology to Investigate the Anti-Alzheimer's Mechanism of Tripterygium Glycoside.

Applications

Unspecified application

Species

Unspecified reactive species

Yongcang Zhang,Quxi Silang,Yan Wang,Niannian Wang,Luobu Gesang,Liang Tang,Lan Liu
View all publications
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