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Rabbit Recombinant Monoclonal NDRG2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 13 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (AB174850), expandable thumbnail
  • Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (AB174850), expandable thumbnail
  • Western blot - Anti-NDRG2 antibody [EPR11910] (AB174850), expandable thumbnail
  • Western blot - Anti-NDRG2 antibody [EPR11910] (AB174850), expandable thumbnail
  • Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (AB174850), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Expected
Tested
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50.00000 - 1/100.00000
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10.00000 - 1/100.00000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Recombinant full length protein - Human
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Mouse
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Rat
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Target data

Function

Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal NDRG2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11910
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

This antibody does not cross-react with human NDRG4.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NDRG2 also known as N-myc downstream-regulated gene 2 is a protein with a molecular mass of approximately 41.5 kDa. It plays an important role in cellular stress responses and cell growth regulation. NDRG2 is typically expressed in various tissues especially in the liver heart and brain. Its expression suggests its involvement in diverse physiological processes which is supported by its presence across multiple cell types.

Biological function summary

NDRG2 influences cellular processes related to oxidative stress and differentiation. It is not known as part of a major protein complex but it interacts with other proteins to modulate cellular environments. NDRG2 acts as a tumor suppressor and can affect cell cycle regulation. It also plays roles in apoptosis and responses to DNA damage essential for maintaining cell health and function.

Pathways

NDRG2 engages in essential cellular processes. It is involved in the PI3K/Akt and MAPK pathways both of which regulate cell proliferation and survival. NDRG2 interacts with proteins such as PTEN and Akt where it can modulate their activities. Through this regulation it impacts cellular responses to growth factors and contributes to maintaining cellular homeostasis.

Associated diseases and disorders

Altered NDRG2 expression links with cancer and neurodegenerative diseases. In cancer its tumor suppressor role involves interactions with proteins such as p53 impacting cell survival pathways. In neurodegenerative diseases changes in NDRG2 expression can affect neuronal cell health and may interact with proteins like tau influencing disease progression. Understanding NDRG2's function helps in exploring therapeutic targets for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (ab174850)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling NDRG2 with purified ab174850 at 1:100 dilution (2.4 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850)

    ab174850 (purified) at 1:20 dilution (2μg) immunoprecipitating NDRG2 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab174850 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab174850 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850)

    Predicted band size: 40 kDa

  • Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850)

    All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution

    All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa

    Observed band size: 41 kDa

  • Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850)

    SW480 and SW620 cells are NDRG2 negative cell lines reported by PMID 23900729

    All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution

    Lane 1: Human brain lysates at 20 µg

    Lane 2: Mouse brain lysates at 20 µg

    Lane 3: Rat brain lysates at 20 µg

    Lane 4: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 5: SW620 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa

    Observed band size: 41 kDa

  • Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850)

    Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1XPBS (negative control) using ab174850, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850)

    Predicted band size: 40 kDa

  • Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850)

    All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Lane 3: Human fetal heart lysate at 10 µg

    Lane 4: Human fetal brain lysate at 10 µg

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (ab174850)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling NDRG2 using ab174850 at 1/50 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (ab174850)

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling NDRG2 using ab174850 at 1/50 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850), expandable thumbnail

    Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as total protein control at 1/5000 dilution.

    This antibody does not cross-react with human NDRG4.

    All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution

    Lane 1: His-tagged human NDRG2 recombinant protein at 10 ng

    Lane 2: His-tagged human NDRG4 recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

    Exposure time: 1s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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