Rabbit Recombinant Monoclonal NDRG2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 13 publications.
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Not recommended | Not recommended |
Mouse | Expected | Expected | Tested | Not recommended | Not recommended |
Rat | Expected | Expected | Tested | Not recommended | Not recommended |
Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Rat | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation.
KIAA1248, SYLD, NDRG2, Protein NDRG2, N-myc downstream-regulated gene 2 protein, Protein Syld709613
Rabbit Recombinant Monoclonal NDRG2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 13 publications.
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
This antibody does not cross-react with human NDRG4.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
NDRG2 also known as N-myc downstream-regulated gene 2 is a protein with a molecular mass of approximately 41.5 kDa. It plays an important role in cellular stress responses and cell growth regulation. NDRG2 is typically expressed in various tissues especially in the liver heart and brain. Its expression suggests its involvement in diverse physiological processes which is supported by its presence across multiple cell types.
NDRG2 influences cellular processes related to oxidative stress and differentiation. It is not known as part of a major protein complex but it interacts with other proteins to modulate cellular environments. NDRG2 acts as a tumor suppressor and can affect cell cycle regulation. It also plays roles in apoptosis and responses to DNA damage essential for maintaining cell health and function.
NDRG2 engages in essential cellular processes. It is involved in the PI3K/Akt and MAPK pathways both of which regulate cell proliferation and survival. NDRG2 interacts with proteins such as PTEN and Akt where it can modulate their activities. Through this regulation it impacts cellular responses to growth factors and contributes to maintaining cellular homeostasis.
Altered NDRG2 expression links with cancer and neurodegenerative diseases. In cancer its tumor suppressor role involves interactions with proteins such as p53 impacting cell survival pathways. In neurodegenerative diseases changes in NDRG2 expression can affect neuronal cell health and may interact with proteins like tau influencing disease progression. Understanding NDRG2's function helps in exploring therapeutic targets for these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling NDRG2 with purified ab174850 at 1:100 dilution (2.4 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
ab174850 (purified) at 1:20 dilution (2μg) immunoprecipitating NDRG2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab174850 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab174850 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850)
Predicted band size: 40 kDa
All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution
All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 41 kDa
SW480 and SW620 cells are NDRG2 negative cell lines reported by PMID 23900729
All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution
Lane 1: Human brain lysates at 20 µg
Lane 2: Mouse brain lysates at 20 µg
Lane 3: Rat brain lysates at 20 µg
Lane 4: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 5: SW620 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 41 kDa
Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1XPBS (negative control) using ab174850, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-NDRG2 antibody [EPR11910] (ab174850)
Predicted band size: 40 kDa
All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: Human fetal kidney lysate at 10 µg
Lane 3: Human fetal heart lysate at 10 µg
Lane 4: Human fetal brain lysate at 10 µg
Predicted band size: 40 kDa
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling NDRG2 using ab174850 at 1/50 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling NDRG2 using ab174850 at 1/50 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as total protein control at 1/5000 dilution.
This antibody does not cross-react with human NDRG4.
All lanes: Western blot - Anti-NDRG2 antibody [EPR11910] (ab174850) at 1/1000 dilution
Lane 1: His-tagged human NDRG2 recombinant protein at 10 ng
Lane 2: His-tagged human NDRG4 recombinant protein at 10 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 1s
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