AB169775

Anti-NDRG2 antibody [EPR11911(B)]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal NDRG2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

KIAA1248, SYLD, NDRG2, Protein NDRG2, N-myc downstream-regulated gene 2 protein, Protein Syld709613

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling NDRG2 with ab169775 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDRG2 with ab169775 at a 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

All lanes:

Western blot - Anti-NDRG2 antibody [EPR11911(B)] (ab169775) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NDRG2 antibody [EPR11911(B)] (ab169775) at 1/1000 dilution

Lane 1:

His-tagged human NDRG2 recombinant protein at 10 ng

Lane 2:

His-tagged human NDRG4 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11911(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody cross-reacts with human NDRG4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDRG2 also known as N-myc downstream-regulated gene 2 is a protein with a molecular mass of approximately 41.5 kDa. It plays an important role in cellular stress responses and cell growth regulation. NDRG2 is typically expressed in various tissues especially in the liver heart and brain. Its expression suggests its involvement in diverse physiological processes which is supported by its presence across multiple cell types.

Biological function summary

NDRG2 influences cellular processes related to oxidative stress and differentiation. It is not known as part of a major protein complex but it interacts with other proteins to modulate cellular environments. NDRG2 acts as a tumor suppressor and can affect cell cycle regulation. It also plays roles in apoptosis and responses to DNA damage essential for maintaining cell health and function.

Pathways

NDRG2 engages in essential cellular processes. It is involved in the PI3K/Akt and MAPK pathways both of which regulate cell proliferation and survival. NDRG2 interacts with proteins such as PTEN and Akt where it can modulate their activities. Through this regulation it impacts cellular responses to growth factors and contributes to maintaining cellular homeostasis.

Altered NDRG2 expression links with cancer and neurodegenerative diseases. In cancer its tumor suppressor role involves interactions with proteins such as p53 impacting cell survival pathways. In neurodegenerative diseases changes in NDRG2 expression can affect neuronal cell health and may interact with proteins like tau influencing disease progression. Understanding NDRG2's function helps in exploring therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation.

See full target information NDRG2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 12:1808-1827 PubMed32003757

2020

ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tianbo Xu,Hailong Ruan,Su Gao,Jingchong Liu,Yuenan Liu,Zhengshuai Song,Qi Cao,Keshan Wang,Lin Bao,Di Liu,Junwei Tong,Jian Shi,Huageng Liang,Hongmei Yang,Ke Chen,Xiaoping Zhang

American journal of obstetrics and gynecology 216:602.e1-602.e21 PubMed28209491

2017

The preterm cervix reveals a transcriptomic signature in the presence of premature prelabor rupture of membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Makieva,Aurelija Dubicke,Sara F Rinaldi,Emma Fransson,Gunvor Ekman-Ordeberg,Jane E Norman

International journal of molecular sciences 17: PubMed27589737

2016

The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jehn-Chuan Lee,Kun-Chun Chiang,Tsui-Hsia Feng,Yu-Jen Chen,Sung-Ting Chuang,Ke-Hung Tsui,Li-Chuan Chung,Horng-Heng Juang

Scientific reports 5:12870 PubMed26249737

2015

Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells.

Applications

Species

Unspecified reactive species

Ke-Hung Tsui,Shu-Yuan Hsu,Li-Chuan Chung,Yu-Hsiang Lin,Tsui-Hsia Feng,Tzu-Yi Lee,Phei-Lang Chang,Horng-Heng Juang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDRG2 antibody [EPR11911(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

Western blot - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

Western blot - Anti-NDRG2 antibody [EPR11911(B)] (AB169775)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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