AB176695

Anti-NDRG3 antibody - C-terminal

Name: Anti-NDRG3 antibody - C-terminal (ab176695) | Abcam
Brand: Abcam Limited
SKU: AB176695
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal NDRG3 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Protein NDRG3 aa 350 to C-terminus.

View Alternative Names

Protein NDRG3, N-myc downstream-regulated gene 3 protein, NDRG3

2 Images

Supplier Data

Immunoprecipitation - Anti-NDRG3 antibody - C-terminal (AB176695)

Immunoprecipitation. Lane 1 : labeling of NDRG3 in HeLa whole cell lysate immunoprecipitated using ab176695 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : control IgG.

All lanes:

Immunoprecipitation - Anti-NDRG3 antibody - C-terminal (ab176695)

Predicted band size: 41 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-NDRG3 antibody - C-terminal (AB176695)

All lanes:

Western blot - Anti-NDRG3 antibody - C-terminal (ab176695) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysates at 50 µg

Lane 2:

HeLa whole cell lysates at 15 µg

Lane 3:

293T whole cell lysates at 50 µg

Lane 4:

Jurkat whole cell lysates at 50 µg

Predicted band size: 41 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human Protein NDRG3 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UGV2

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDRG3 also known as N-Myc downstream-regulated gene 3 is a protein that acts as a stress-responsive molecule. Its molecular mass is approximately 41 kDa. Mechanically NDRG3 functions by interacting with other cellular pathways to ensure a proper stress response. The protein is widely expressed in various tissues with notable expression in the heart skeletal muscle and kidney. NDRG3 plays significant roles in cellular processes by modulating pathways in response to changes in the oxygen levels and nutrient availability.

Biological function summary

NDRG3 participates in processes that involve cell proliferation and differentiation and it can influence tumorigenic behavior. It is not part of a complex but interacts with other signaling molecules to exert its biological functions. NDRG3 responds to hypoxic conditions by stabilizing hypoxia-inducible factor (HIF)-1α promoting cell survival and adaptation under low oxygen availability. This capability makes it an important factor in cell adaptability under stress conditions.

Pathways

Four significant ones connect to NDRG3's involvement including the hypoxia response pathway and the MAPK/ERK signaling pathway. NDRG3 interacts closely with proteins like HIF-1α within the hypoxia response. In the MAPK/ERK pathway it influences downstream signals impacting cellular growth and differentiation. These interactions suggest that NDRG3 is integral to maintaining cellular homeostasis in environmental stress scenarios.

NDRG3 links to cancer progression and ischemic disorders. Its involvement in stabilizing HIF-1α connects it to cancer as the protein supports tumor growth and metastatic potential under hypoxic conditions often found in tumors. Similarly it relates to ischemic conditions in tissues like the heart where it helps regulate cellular responses to oxygen deprivation. Connections to proteins like HIF-1α in these cases highlight NDRG3's potential as a therapeutic target in managing these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Protein NDRG3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 14:1319785 PubMed38098676

2023

Cranberry, but not D-mannose and ibuprofen, prevents against uropathogenic -induced cell damage and cell death in MDCK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jenane Konesan,Jenny Wang,Kate H Moore,Kylie J Mansfield,Lu Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDRG3 antibody - C-terminal

Immunoprecipitation - Anti-NDRG3 antibody - C-terminal (AB176695)

Western blot - Anti-NDRG3 antibody - C-terminal (AB176695)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise