AB131266

Anti-NDRG3 antibody [EPR9010(B)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal NDRG3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Protein NDRG3, N-myc downstream-regulated gene 3 protein, NDRG3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling NDRG3 with ab131266 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling NDRG3 with ab131266 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

All lanes:

Western blot - Anti-NDRG3 antibody [EPR9010(B)] (ab131266) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

LNCaP cell lysate at 10 µg

Predicted band size: 41 kDa

false

OI-RD Scanning - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9010(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDRG3 also known as N-Myc downstream-regulated gene 3 is a protein that acts as a stress-responsive molecule. Its molecular mass is approximately 41 kDa. Mechanically NDRG3 functions by interacting with other cellular pathways to ensure a proper stress response. The protein is widely expressed in various tissues with notable expression in the heart skeletal muscle and kidney. NDRG3 plays significant roles in cellular processes by modulating pathways in response to changes in the oxygen levels and nutrient availability.

Biological function summary

NDRG3 participates in processes that involve cell proliferation and differentiation and it can influence tumorigenic behavior. It is not part of a complex but interacts with other signaling molecules to exert its biological functions. NDRG3 responds to hypoxic conditions by stabilizing hypoxia-inducible factor (HIF)-1α promoting cell survival and adaptation under low oxygen availability. This capability makes it an important factor in cell adaptability under stress conditions.

Pathways

Four significant ones connect to NDRG3's involvement including the hypoxia response pathway and the MAPK/ERK signaling pathway. NDRG3 interacts closely with proteins like HIF-1α within the hypoxia response. In the MAPK/ERK pathway it influences downstream signals impacting cellular growth and differentiation. These interactions suggest that NDRG3 is integral to maintaining cellular homeostasis in environmental stress scenarios.

NDRG3 links to cancer progression and ischemic disorders. Its involvement in stabilizing HIF-1α connects it to cancer as the protein supports tumor growth and metastatic potential under hypoxic conditions often found in tumors. Similarly it relates to ischemic conditions in tissues like the heart where it helps regulate cellular responses to oxygen deprivation. Connections to proteins like HIF-1α in these cases highlight NDRG3's potential as a therapeutic target in managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Protein NDRG3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eads5143 PubMed40073135

2025

Ndrg3 is a critical regulator of peripheral T cell maturation and homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Komorowska,Christiane Grammer,Mirela Bălan,Jeremy B Swann

Experimental and therapeutic medicine 24:622 PubMed36160904

2022

LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoliang Luo,Tingliang Gong,Weihong Li,Wenqiang Tao

International journal of molecular sciences 17: PubMed27589737

2016

The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jehn-Chuan Lee,Kun-Chun Chiang,Tsui-Hsia Feng,Yu-Jen Chen,Sung-Ting Chuang,Ke-Hung Tsui,Li-Chuan Chung,Horng-Heng Juang

Scientific reports 5:12870 PubMed26249737

2015

Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells.

Applications

Species

Unspecified reactive species

Ke-Hung Tsui,Shu-Yuan Hsu,Li-Chuan Chung,Yu-Hsiang Lin,Tsui-Hsia Feng,Tzu-Yi Lee,Phei-Lang Chang,Horng-Heng Juang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDRG3 antibody [EPR9010(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

Western blot - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

OI-RD Scanning - Anti-NDRG3 antibody [EPR9010(B)] (AB131266)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise