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AB191613

Anti-NDST2 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal NDST2 antibody. N-terminal. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NDST2.

View Alternative Names

HSST2, NDST2, Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2, Glucosaminyl N-deacetylase/N-sulfotransferase 2, N-heparan sulfate sulfotransferase 2, NDST-2, N-HSST 2

1 Images
Western blot - Anti-NDST2 antibody - N-terminal (AB191613)
  • WB

Supplier Data

Western blot - Anti-NDST2 antibody - N-terminal (AB191613)

All lanes:

Western blot - Anti-NDST2 antibody - N-terminal (ab191613) at 1 µg/mL

Lane 1:

A20 cell lysate at 15 µg

Lane 2:

A20 cell lysate at 15 µg with blocking peptide

Predicted band size: 101 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NDST2. The exact immunogen used to generate this antibody is proprietary information.

P52849

Specificity

At least two isoforms of NDST2 are known to exist; ab191613 will only detect the larger isoform. ab191613 is predicted not to cross-react with other members of the NDST protein family.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab191613 is affinity chromatography purified via peptide column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDST2 also known as N-deacetylase/N-sulfotransferase 2 is an enzyme with a mass of roughly 100 kDa. This enzyme participates in the modification of heparan sulfate proteoglycans by catalyzing the N-deacetylation and subsequent N-sulfation of glucosamine residues. NDST2 expression occurs widely in many tissues including the brain kidney and lung. Its activity is necessary for the maturation of heparan sulfate chains which play important roles in various cellular processes.
Biological function summary

NDST2 modifies heparan sulfate proteoglycans which affects cell signaling and interactions. This enzyme functions independently but its product the modified heparan sulfate interacts with other molecules to form complex networks essential for biological activities. NDST2’s activity impacts processes such as growth factor signaling and extracellular matrix organization influencing cell growth and communication. Its role is key in regulating biological pathways through modifying heparan sulfate chains which influences the availability of signaling molecules.

Pathways

NDST2 plays a role in the heparan sulfate biosynthesis and signaling pathways. It interacts with proteins like fibroblast growth factor receptors by modifying heparan sulfate’s structure. This modification is important for the fibrogen and Wnt signaling pathways. Through these pathways NDST2 regulates processes such as development proliferation and tissue repair by facilitating ligand-receptor interactions.

NDST2 involvement is observed in conditions like cancer and hereditary multiple exostoses (HME). Aberrant NDST2 function can lead to improper heparan sulfate biosynthesis affecting tumor progression and metastasis in cancer. In HME altered NDST2 activity modifies cartilage growth. Interactions with proteins like EXT1 and EXT2 which are also involved in heparan sulfate biosynthesis highlight NDST2’s relevance in these disorders. Studying NDST2 further may offer insights into therapeutic targets for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413).
See full target information NDST2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0332202 PubMed40991530

2025

Investigating the anti-osteosarcoma effects of Patchouli alcohol through protein network mapping and in vitro experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyu Zhan,He Pang,Hang Wu,Lijun Song,Bo Wei
View all publications
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