Anti-NDUAB antibody [EPR15251]

• WB

Supplier Data

Western blot - Anti-NDUAB antibody [EPR15251] (AB183707)

All lanes:

Western blot - Anti-NDUAB antibody [EPR15251] (ab183707) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human skeletal lysate at 20 µg

Lane 3:

Y79 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 15 kDa,41 kDa

Observed band size: 13 kDa

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• WB

CiteAb

Western blot - Anti-NDUAB antibody [EPR15251] (AB183707)

NDUAB western blot using anti-NDUAB antibody [EPR15251] ab183707. Publication image and figure legend from Kim, M. J., Serwa, R. A., et al., 2023, Nat Commun, PubMed 37433777.

ab183707 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183707 please see the product overview.

Small HSP and HSP110 families have a tendency to be increased under mitochondrial stress.a RNA-seq analysis of HSPs gene expression log2 fold changes (log2FC) in NDUFA11 KO and NDUFA13 KO compared to WT HEK293T cells (n = 4). Up- and down-regulated genes (q-value < 0.05) are shown in green and pink, respectively. The intensity of the color shades depends on the level of expression change. Gray indicates genes with not statistically significant expression changes. b mRNA expression patterns of selected transcripts validated by RT-qPCR. The mRNA levels are presented as fold changes relative to WT. Data shown are mean ± SD (n = 3 biological replicates with two technical replicates). p-value from an ordinary one-way ANOVA with Dunnett's multiple comparisons test using GraphPad Prism. c Western blot analysis of HSPs expression performed in whole cell lysates of NDUFA11 KO, NDUFA13 KO and WT HEK293T cells. ACTB was used as a loading control. Data shown are representative of three independent experiments. d Quantification of HSPs in western blot analysis normalized to ACTB using ImageJ. The protein levels are presented as fold changes relative to WT. Data shown are mean ± SD (n = 3). p-value from two-sided, unpaired t-test using GraphPad Prism. Source data are provided as a Source Data file.

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