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AB250684

Anti-NDUAB antibody [EPR15251] - BSA and Azide free

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Rabbit Recombinant Monoclonal NDUAB antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11, Complex I-B14.7, NADH-ubiquinone oxidoreductase subunit B14.7, CI-B14.7, NDUFA11

1 Images
Western blot - Anti-NDUAB antibody [EPR15251] - BSA and Azide free (AB250684)
  • WB

Supplier Data

Western blot - Anti-NDUAB antibody [EPR15251] - BSA and Azide free (AB250684)

This data was developed using ab183707, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUAB antibody [EPR15251] (<a href='/en-us/products/primary-antibodies/nduab-antibody-epr15251-ab183707'>ab183707</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human skeletal lysate at 20 µg

Lane 3:

Y79 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 15 kDa,41 kDa

Observed band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15251

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250684 is the carrier-free version of ab183707.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUAB also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 is an important component of the mitochondrial electron transport chain. This protein has a mass of approximately 14 kDa and plays an essential role in cellular respiration by facilitating the transfer of electrons. It is expressed in the mitochondria across various cell types enabling the production of ATP the energy currency of cells.
Biological function summary

NDUAB is part of a larger complex specifically Complex I also called NADH:ubiquinone oxidoreductase. This complex is the first enzyme of the mitochondrial electron transport chain and is responsible for catalyzing the transfer of electrons from NADH to ubiquinone. NDUAB supports the electron transfer mechanism allowing for the establishment of a proton gradient across the mitochondrial membrane. The proton gradient is vital for ATP synthesis through oxidative phosphorylation.

Pathways

NDUAB's function plays an important role in the oxidative phosphorylation pathway. This pathway is important for the energy metabolism of aerobic organisms and links to the citric acid cycle. NDUAB collaborates with other proteins in Complex I like NDUFS1 and NDUFA10 to facilitate electron transport and proton pumping necessary for ATP production. The proper function of this process is essential for sustaining cellular energy levels.

NDUAB has been linked to mitochondrial diseases such as Leigh syndrome and mitochondrial encephalomyopathy. These disorders typically result from dysfunctions in mitochondrial complex I where NDUAB and related proteins such as NDUFS4 and NDUFAF2 may be involved. These diseases often lead to neurological and muscular symptoms due to impaired ATP production highlighting the importance of NDUAB in maintaining cellular energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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