AB122270

Anti-NDUF3 antibody

Name: Anti-NDUF3 antibody (ab122270) | Abcam
Brand: Abcam Limited
SKU: AB122270
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NDUF3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NDUFAF3 aa 100-200.

View Alternative Names

C3orf60, NDUFAF3, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (AB122270)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (AB122270)

ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human prostate tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (AB122270)

ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (AB122270)

ab122270, at 1/50 dilution, staining NDUF3 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

Unknown

Western blot - Anti-NDUF3 antibody (AB122270)

All lanes:

Western blot - Anti-NDUF3 antibody (ab122270) at 1/250 dilution

Lane 1:

RT-4 lysate

Lane 2:

U251 -MG lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NDUFAF3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BU61

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUF3 also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 plays an essential role in the electron transport chain within mitochondria. This protein has a molecular mass of about 22 kDa. You can usually find NDUF3 expression in tissues with high metabolic demands such as the brain heart and skeletal muscles. It forms a part of the mitochondrial complex I which drives the initial steps of cellular respiration.

Biological function summary

NDUF3 assists in the proper functioning of complex I within the respiratory chain. As part of this complex it contributes to electron transfer from NADH to ubiquinone. This protein participates in maintaining an efficient oxidative phosphorylation process which is essential for ATP synthesis. Along with other subunits NDUF3 helps maintain the structural integrity and activity of complex I influencing the overall energy production capacity of cells.

Pathways

Oxidative phosphorylation and electron transport chains involve NDUF3 as a significant component. This protein makes connections with other complex I subunits like NDUFS1 and NDUFB8 to facilitate proper electron transfer and proton gradient across the mitochondrial membrane. These pathways are vital to cellular energy metabolism and involve complex interactions with different proteins within the electron transport chain.

Mutations or dysfunctions in NDUF3 have links to mitochondrial diseases including Leigh syndrome. This protein has associations with neurodegenerative disorders due to its role in maintaining mitochondrial function. NDUF3 disruptions affect energy production leading to cellular damage. Moreover its interactions with proteins like NDUFS1 have a strong connection to metabolic disorders signaling the importance of NDUF3 in maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential factor for the assembly of mitochondrial NADH : ubiquinone oxidoreductase complex (complex I).

See full target information NDUFAF3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:1045-1063 PubMed38322121

2024

The homeoprotein HOXB2 limits triple-negative breast carcinogenesis via extracellular matrix remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hoon Oh,Clara Yuri Kim,Da Som Jeong,Yu Cheon Kim,Myoung Hee Kim,Je-Yoel Cho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (AB122270)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (AB122270)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUF3 antibody (AB122270)

Western blot - Anti-NDUF3 antibody (AB122270)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise