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AB176563

Anti-NDUFA1 antibody [EPR12083]

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(5 Publications)

Rabbit Recombinant Monoclonal NDUFA1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, Complex I-MWFE, NADH-ubiquinone oxidoreductase MWFE subunit, CI-MWFE, NDUFA1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (AB176563)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (AB176563)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human heart tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-NDUFA1 antibody [EPR12083] (AB176563)
  • WB

Supplier Data

Western blot - Anti-NDUFA1 antibody [EPR12083] (AB176563)

All lanes:

Western blot - Anti-NDUFA1 antibody [EPR12083] (ab176563) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Human skeletal muscle tissue lysate at 10 µg

Lane 4:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 8 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (AB176563)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (AB176563)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human skeletal muscle tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] (AB176563)
  • IP

Supplier Data

Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] (AB176563)

Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling NDUFA1 with ab176563 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] (ab176563)

Predicted band size: 8 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12083

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Gorilla": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA1 also called NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 is a critical component of the mitochondrial respiratory chain. It has an approximate molecular mass of 5.5 kDa and resides within the inner mitochondrial membrane. This protein is part of the larger NADH:ubiquinone oxidoreductase also known as Complex I which is present across various tissues where energy production is essential. NDUFA1 aids the proper function of Complex I to facilitate electron transfer during cellular respiration.
Biological function summary

NDUFA1 plays an integral role in the electron transfer process by linking with other subunits to form the Complex I assembly. It is important for the initial step of the electron transport chain where it helps in transferring electrons from NADH to ubiquitin. This step is important as it contributes to the generation of a proton gradient across the mitochondrial membrane which is necessary for ATP synthesis. Part of the mitochondrial enzymatic pathway NDUFA1 works in concert with other enzyme complexes to maintain cellular energy balance.

Pathways

NDUFA1 engages primarily in the oxidative phosphorylation pathway which is pivotal for ATP production in cells. This pathway connects NDUFA1 with other complexes such as Complex II Complex III and Complex IV ensuring comprehensive electron flow and proton gradient creation. In addition NDUFA1 is involved in the mitochondrial electron transport chain linking with proteins such as cytochrome c for maintaining energy metabolism and cellular respiration.

Mutations or dysfunctions in NDUFA1 relate to mitochondrial disorders like Leigh syndrome which affect energy-dependent tissues such as the brain and muscles. Such mutations lead to impaired electron transfer therefore causing energy deficits. NDUFA1 mutations can also contribute to cardiac and neurological disorders due to disrupted ATP synthesis. Within these diseases NDUFA1 interacts with other defective proteins of the mitochondrial respiratory chain exacerbating metabolic irregularities and contributing to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFA1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:1718-1731 PubMed40891247

2025

LINC00114 promotes colorectal cancer metastasis by targeting HNRNPA1 to regulate glutamine metabolism reprogramming and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Tang,Meng Liu,Yangyang Zhu,Lifa Li,Chen Qing,Yuehua Guan,Tong Zhou,Xuegui Tang

Scientific reports 15:10925 PubMed40157968

2025

Characterization of a new mutation of mitochondrial ND6 gene in hepatocellular carcinoma and its effects on respiratory complex I.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Bazzani,Deepali L Kundnani,Mara Equisoain Redin,Francesca Agostini,Kirti Chhatlani,Angelo Corso Faini,Jakub Poziemski,Umberto Baccarani,Pawel Siedlecki,Silvia Deaglio,Francesca Storici,Carlo Vascotto

RNA (New York, N.Y.) 30:223-239 PubMed38164626

2024

LARP4 is an RNA-binding protein that binds nuclear-encoded mitochondrial mRNAs to promote mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin M Lewis,Chae Yun Cho,Hsuan-Lin Her,Orel Mizrahi,Tony Hunter,Gene W Yeo

Scientific reports 11:17129 PubMed34429458

2021

Decreased PGC1β expression results in disrupted human erythroid differentiation, impaired hemoglobinization and cell cycle exit.

Applications

Unspecified application

Species

Unspecified reactive species

Taha Sen,Jun Chen,Sofie Singbrant

Cell death and differentiation 27:1660-1676 PubMed31685978

2019

Epigenetic regulation of the Warburg effect by H2B monoubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Ya Jing,Feng-Feng Cai,Lei Zhang,Jing Han,Lu Yang,Fan Tang,Ya-Bin Li,Jian-Feng Chang,Feng Sun,Xiao-Mei Yang,Fang-Lin Sun,Su Chen
View all publications
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Product promise

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