AB249923

Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal NDUFA1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, Complex I-MWFE, NADH-ubiquinone oxidoreductase MWFE subunit, CI-MWFE, NDUFA1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

This data was developed using ab176563, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human skeletal muscle tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

This data was developed using ab176563, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

This data was developed using ab176563, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling NDUFA1 with ab176563 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] (<a href='/en-us/products/primary-antibodies/ndufa1-antibody-epr12083-ab176563'>ab176563</a>)

Predicted band size: 8 kDa

true

Supplier Data

Western blot - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

This data was developed using ab176563, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFA1 antibody [EPR12083] (<a href='/en-us/products/primary-antibodies/ndufa1-antibody-epr12083-ab176563'>ab176563</a>) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Human skeletal muscle tissue lysate at 10 µg

Lane 4:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 8 kDa

true

Unconjugated

Anti-NDUFA1 antibody [EPR12083]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12083

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249923 is the carrier-free version of ab176563.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA1 also called NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 is a critical component of the mitochondrial respiratory chain. It has an approximate molecular mass of 5.5 kDa and resides within the inner mitochondrial membrane. This protein is part of the larger NADH:ubiquinone oxidoreductase also known as Complex I which is present across various tissues where energy production is essential. NDUFA1 aids the proper function of Complex I to facilitate electron transfer during cellular respiration.

Biological function summary

NDUFA1 plays an integral role in the electron transfer process by linking with other subunits to form the Complex I assembly. It is important for the initial step of the electron transport chain where it helps in transferring electrons from NADH to ubiquitin. This step is important as it contributes to the generation of a proton gradient across the mitochondrial membrane which is necessary for ATP synthesis. Part of the mitochondrial enzymatic pathway NDUFA1 works in concert with other enzyme complexes to maintain cellular energy balance.

Pathways

NDUFA1 engages primarily in the oxidative phosphorylation pathway which is pivotal for ATP production in cells. This pathway connects NDUFA1 with other complexes such as Complex II Complex III and Complex IV ensuring comprehensive electron flow and proton gradient creation. In addition NDUFA1 is involved in the mitochondrial electron transport chain linking with proteins such as cytochrome c for maintaining energy metabolism and cellular respiration.

Mutations or dysfunctions in NDUFA1 relate to mitochondrial disorders like Leigh syndrome which affect energy-dependent tissues such as the brain and muscles. Such mutations lead to impaired electron transfer therefore causing energy deficits. NDUFA1 mutations can also contribute to cardiac and neurological disorders due to disrupted ATP synthesis. Within these diseases NDUFA1 interacts with other defective proteins of the mitochondrial respiratory chain exacerbating metabolic irregularities and contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NDUFA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

Molecular cancer 20:123 PubMed34579723

2021

p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yang,Anpei Hu,Yanhua Guo,Jianqun Wang,Dan Li,Xiaojing Wang,Shikai Jin,Boling Yuan,Shuang Cai,Yi Zhou,Qilan Li,Guo Chen,Haiyang Gao,Liduan Zheng,Qiangsong Tong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

Western blot - Anti-NDUFA1 antibody [EPR12083] - BSA and Azide free (AB249923)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise