AB129752

Anti-NDUFA4 antibody

Name: Anti-NDUFA4 antibody (ab129752) | Abcam
Brand: Abcam Limited
SKU: AB129752

Be the first to review this product! Submit a review

(15 Publications)

Rabbit Polyclonal NDUFA4 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4.

View Alternative Names

Cytochrome c oxidase subunit NDUFA4, Complex I-MLRQ, NADH-ubiquinone oxidoreductase MLRQ subunit, CI-MLRQ, NDUFA4

1 Images

Unknown

Western blot - Anti-NDUFA4 antibody (AB129752)

All lanes:

Western blot - Anti-NDUFA4 antibody (ab129752) at 1/100 dilution

Lane 1:

HepG2 cell extract

Lane 2:

HepG2 cell extract with immunizing peptide

Lane 3:

RAW264.7 cell extract

Predicted band size: 9 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, ELISA

applications

Immunogen

Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4. The exact immunogen used to generate this antibody is proprietary information.

O00483

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA4 also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 is a small protein with a molecular mass of around 9 kDa. It plays a role as part of the mitochondrial respiratory chain where it contributes to the electron transport process. NDUFA4 is expressed in various tissues but has higher levels of expression in tissues with elevated metabolic demand like the heart liver and muscle. Its location in the inner mitochondrial membrane allows it to participate directly in respiration activity by aiding electron transfer from NADH to ubiquinone.

Biological function summary

NDUFA4 functions as part of the larger protein complex known as Complex I or NADH:ubiquinone oxidoreductase. Complex I is a critical component of the mitochondrial electron transport chain. It supports ATP synthesis by creating a proton gradient across the inner mitochondrial membrane. NDUFA4 participates in this energy conversion process and cooperates with additional protein subunits in Complex I to maintain cellular energy metabolism. This makes it essential for maintaining efficient aerobic respiration.

Pathways

NDUFA4 has a significant role within the oxidative phosphorylation pathway a central metabolic pathway in cellular energy production. Within this pathway it associates with other proteins such as NDUFB8 and NDUFS1 in Complex I which work together for effective electron transport. NDUFA4 also intersects with the broader pathways of metabolic regulation lending support to the processes that produce ATP as a cellular energy currency.

Mutations or dysregulation of NDUFA4 have been connected to mitochondrial diseases such as mitochondrial myopathy. These conditions arise due to impaired energy production in cells. Additionally alterations in NDUFA4 levels or activity have implications in neurodegenerative disorders like Parkinson's disease. In such diseases proteins like NDUFS4 another Complex I subunit also become affected highlighting potential protein-protein interactions critical for maintaining mitochondrial health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (PubMed : 22902835). NDUFA4 is required for complex IV maintenance (PubMed : 22902835).

See full target information Cytochrome c oxidase subunit NDUFA4

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40956617

2025

miR147 promotes mucosal integrity and healing in intestinal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka K Czopik,Arash Dabiri,Chia-Hao Tung,Victoria Vaughn,Xiangsheng Huang,Jinlian Wang,Hui Li,Nicolas F Moreno,Natalia V Piwko,Katherine Figarella,Hongfang Liu,Zhongming Zhao,Xiaoyi Yuan,Holger K Eltzschig

Functional & integrative genomics 25:90 PubMed40240625

2025

Identification and mechanistic analysis of shared biomarkers and pathogenesis in acute pancreatitis and sepsis based on differential gene expression and protein interaction networks.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Lu,Yifeng Mao,Shangwen Cai,Qingqing Chen,Panpan Xu,Chenghua Xu,Cheng Zheng,Jian Lan

iScience 26:107001 PubMed37534130

2023

Isogenic human trophectoderm cells demonstrate the role of and associated variants in ZIKV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Liuliu Yang,Yuling Han,Ting Zhou,Lauretta A Lacko,Mohsan Saeed,Christina Tan,Ron Danziger,Jiajun Zhu,Zeping Zhao,Clare Cahir,Alice Maria Giani,Yang Li,Xue Dong,Dorota Moroziewicz,Daniel Paull,Zhengming Chen,Aaron Zhong,Scott A Noggle,Charles M Rice,Qibin Qi,Todd Evans,Shuibing Chen

Journal of the American Society of Nephrology : JASN 34:1381-1397 PubMed37211637

2023

miR-147 Represses NDUFA4, Inducing Mitochondrial Dysfunction and Tubular Damage in Cold Storage Kidney Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiefu Zhu,Xiaohong Xiang,Xiaoru Hu,Chenglong Li,Zhixia Song,Zheng Dong

Molecular neurobiology 60:2986-3003 PubMed36763283

2023

Ndufa4 Regulates the Proliferation and Apoptosis of Neurons via miR-145a-5p/Homer1/Ccnd2.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Fu,Chen Chen,Kun Du,Lu-Shan Li,Ru Li,Ting-Ying Lei,Qiong Deng,Dan Wang,Qiu-Xia Yu,Xin Yang,Jin Han,Min Pan,Li Zhen,Li-Na Zhang,Jian Li,Fa-Tao Li,Yong-Ling Zhang,Xiang-Yi Jing,Fu-Cheng Li,Dong-Zhi Li,Can Liao

Cell stem cell 29:1475-1490.e6 PubMed36206731

2022

A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants.

Applications

Unspecified application

Species

Unspecified reactive species

Yuling Han,Lei Tan,Ting Zhou,Liuliu Yang,Lucia Carrau,Lauretta A Lacko,Mohsan Saeed,Jiajun Zhu,Zeping Zhao,Benjamin E Nilsson-Payant,Filipe Tenorio Lira Neto,Clare Cahir,Alice Maria Giani,Jin Chou Chai,Yang Li,Xue Dong,Dorota Moroziewicz,Daniel Paull,Tuo Zhang,Soyeon Koo,Christina Tan,Ron Danziger,Qian Ba,Lingling Feng,Zhengming Chen,Aaron Zhong,Gilbert J Wise,Jenny Z Xiang,Hui Wang,Robert E Schwartz,Benjamin R tenOever,Scott A Noggle,Charles M Rice,Qibin Qi,Todd Evans,Shuibing Chen

Cell death & disease 13:715 PubMed35977935

2022

m6A RNA methylation-mediated NDUFA4 promotes cell proliferation and metabolism in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weihong Xu,Yanan Lai,Yunqi Pan,Meiyu Tan,Yanyun Ma,Huiming Sheng,Jiucun Wang

Life (Basel, Switzerland) 12: PubMed36013387

2022

Tunicamycin-Induced Endoplasmic Reticulum Stress Damages Complex I in Cardiac Mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Chen,Jeremy Thompson,Ying Hu,Edward J Lesnefsky

Translational oncology 14:101146 PubMed34118692

2021

High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Wang,Hongsai Chen,Lu Xue,Weiwei He,Wenying Shu,Hao Wu,Zhaoyan Wang

Journal of the European Academy of Dermatology and Venereology : JEADV 35:1393-1406 PubMed33428294

2021

Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.

Applications

Unspecified application

Species

Unspecified reactive species

T Yuan,M-L Cai,Y-M Sheng,X Ding,T-T Shen,W-R Li,H Huang,B Liang,X-J Zhang,Q-X Zhu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFA4 antibody

Western blot - Anti-NDUFA4 antibody (AB129752)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise