Rabbit Polyclonal NDUFA4 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4.
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- Molecular neurobiology 60:2986-30032023Ndufa4 Regulates the Proliferation and Apoptosis of Neurons via miR-145a-5p/Homer1/Ccnd2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Fu,Chen Chen,Kun Du,Lu-Shan Li,Ru Li,Ting-Ying Lei,Qiong Deng,Dan Wang,Qiu-Xia Yu,Xin Yang,Jin Han,Min Pan,Li Zhen,Li-Na Zhang,Jian Li,Fa-Tao Li,Yong-Ling Zhang,Xiang-Yi Jing,Fu-Cheng Li,Dong-Zhi Li,Can LiaoPubMed 36763283
- Cell stem cell 29:1475-1490.e62022A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuling Han,Lei Tan,Ting Zhou,Liuliu Yang,Lucia Carrau,Lauretta A Lacko,Mohsan Saeed,Jiajun Zhu,Zeping Zhao,Benjamin E Nilsson-Payant,Filipe Tenorio Lira Neto,Clare Cahir,Alice Maria Giani,Jin Chou Chai,Yang Li,Xue Dong,Dorota Moroziewicz,Daniel Paull,Tuo Zhang,Soyeon Koo,Christina Tan,Ron Danziger,Qian Ba,Lingling Feng,Zhengming Chen,Aaron Zhong,Gilbert J Wise,Jenny Z Xiang,Hui Wang,Robert E Schwartz,Benjamin R tenOever,Scott A Noggle,Charles M Rice,Qibin Qi,Todd Evans,Shuibing ChenPubMed 36206731
- Life (Basel, Switzerland) 12:2022Tunicamycin-Induced Endoplasmic Reticulum Stress Damages Complex I in Cardiac Mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQun Chen,Jeremy Thompson,Ying Hu,Edward J LesnefskyPubMed 36013387
- Translational oncology 14:1011462021High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhigang Wang,Hongsai Chen,Lu Xue,Weiwei He,Wenying Shu,Hao Wu,Zhaoyan WangPubMed 34118692
- Journal of the European Academy of Dermatology and Venereology : JEADV 35:1393-14062021Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesT Yuan,M-L Cai,Y-M Sheng,X Ding,T-T Shen,W-R Li,H Huang,B Liang,X-J Zhang,Q-X ZhuPubMed 33428294
- Archives of biochemistry and biophysics 683:1082992020Endoplasmic reticulum stress-induced complex I defect: Central role of calcium overload.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed A Mohsin et. al.PubMed 32061585
- Biochemical and biophysical research communications 506:251-2582018LncRNA MAFG-AS1 promotes the progression of colorectal cancer by sponging miR-147b and activation of NDUFA4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Cui,Xi Yang,Lihong Zhang,Yi Zhao,Weiqun YanPubMed 30348529
- The EMBO journal 37:2018Infiltrative and drug-resistant slow-cycling cells support metabolic heterogeneity in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan B Hoang-Minh et. al.PubMed 30322894
- The Journal of clinical investigation 127:1856-18722017Folate cycle enzyme MTHFD1L confers metabolic advantages in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek Lee et. al.PubMed 28394261
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4. The exact immunogen used to generate this antibody is proprietary information. Database link O00483
Reactivity data
Select an application| ELISA | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Expected | Predicted | Expected |
| Rat | Predicted | Predicted | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (PubMed:22902835). NDUFA4 is required for complex IV maintenance (PubMed:22902835).
Alternative names
Cytochrome c oxidase subunit NDUFA4, Complex I-MLRQ, NADH-ubiquinone oxidoreductase MLRQ subunit, CI-MLRQ, NDUFA4
Recommended products
Rabbit Polyclonal NDUFA4 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4. The exact immunogen used to generate this antibody is proprietary information. Database link O00483
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NDUFA4 also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 is a small protein with a molecular mass of around 9 kDa. It plays a role as part of the mitochondrial respiratory chain where it contributes to the electron transport process. NDUFA4 is expressed in various tissues but has higher levels of expression in tissues with elevated metabolic demand like the heart liver and muscle. Its location in the inner mitochondrial membrane allows it to participate directly in respiration activity by aiding electron transfer from NADH to ubiquinone.
- Biological function summary
NDUFA4 functions as part of the larger protein complex known as Complex I or NADH:ubiquinone oxidoreductase. Complex I is a critical component of the mitochondrial electron transport chain. It supports ATP synthesis by creating a proton gradient across the inner mitochondrial membrane. NDUFA4 participates in this energy conversion process and cooperates with additional protein subunits in Complex I to maintain cellular energy metabolism. This makes it essential for maintaining efficient aerobic respiration.
- Pathways
NDUFA4 has a significant role within the oxidative phosphorylation pathway a central metabolic pathway in cellular energy production. Within this pathway it associates with other proteins such as NDUFB8 and NDUFS1 in Complex I which work together for effective electron transport. NDUFA4 also intersects with the broader pathways of metabolic regulation lending support to the processes that produce ATP as a cellular energy currency.
- Associated diseases and disorders
Mutations or dysregulation of NDUFA4 have been connected to mitochondrial diseases such as mitochondrial myopathy. These conditions arise due to impaired energy production in cells. Additionally alterations in NDUFA4 levels or activity have implications in neurodegenerative disorders like Parkinson's disease. In such diseases proteins like NDUFS4 another Complex I subunit also become affected highlighting potential protein-protein interactions critical for maintaining mitochondrial health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-NDUFA4 antibody (ab129752)
All lanes: Western blot - Anti-NDUFA4 antibody (ab129752) at 1/100 dilution
Lane 1: HepG2 cell extract
Lane 2: HepG2 cell extract with immunizing peptide
Lane 3: RAW264.7 cell extract
Predicted band size: 9 kDa
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