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AB241492

Anti-NDUFA5 antibody

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(1 Publication)

Rabbit Polyclonal NDUFA5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NDUFA5 aa 50 to C-terminus.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, Complex I subunit B13, Complex I-13kD-B, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, CI-13kD-B, NDUFA5

2 Images
Western blot - Anti-NDUFA5 antibody (AB241492)
  • WB

Supplier Data

Western blot - Anti-NDUFA5 antibody (AB241492)

Prepared using NETN lysis buffer

All lanes:

Western blot - Anti-NDUFA5 antibody (ab241492) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Predicted band size: 13 kDa

true

Exposure time: 30s

Immunoprecipitation - Anti-NDUFA5 antibody (AB241492)
  • IP

Supplier Data

Immunoprecipitation - Anti-NDUFA5 antibody (AB241492)

NDUFA5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241492 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab241492 at 0.4 μg/ml.

Lane 1 : ab241492 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 75 seconds.

All lanes:

Immunoprecipitation - Anti-NDUFA5 antibody (ab241492)

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NDUFA5 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16718

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to NDUFA5 immobilized on solid support
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA5 also known as NADH:Ubiquinone Oxidoreductase Subunit A5 is part of the mitochondrial respiratory chain complex I. This protein has an approximate molecular mass of 15 kDa. NDUFA5 is expressed in many tissues but shows higher levels in energy-demanding organs such as the heart brain and skeletal muscle. It contributes to cellular respiration by assisting in electron transfer from NADH to ubiquinone which is critical in energy production.
Biological function summary

NDUFA5 is an essential component of Complex I in the mitochondrial inner membrane. Complex I the largest enzyme complex of the mitochondrial electron transport chain consists of 45 subunits. NDUFA5 functions by aiding electron transfer through this complex. Proper function of NDUFA5 is vital for ATP generation through oxidative phosphorylation an important process in bioenergetics.

Pathways

The role of NDUFA5 in the electron transport chain places it central to the oxidative phosphorylation pathway. This pathway also includes other complexes such as Complex III and IV which continue electron transfer leading to ATP synthesis. NDUFA5 interacts closely with other subunits within Complex I like NDUFS2 and forms a luminal proton gradient necessary for ATP production.

NDUFA5 mutations have links to mitochondrial Complex I deficiency a condition causing disorders in energy metabolism. This deficiency can lead to a wide range of clinical manifestations including Leigh syndrome a severe neurological disorder. NDUFA5 dysfunction affects energy-intensive tissues and its abnormal activity could involve other proteins in Complex I such as NDUFS4 which together contribute to the pathology of these mitochondrial disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFA5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:656 PubMed37740194

2023

Activation of CTNNB1 by deubiquitinase UCHL3-mediated stabilization facilitates bladder cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Meng-Qi Fan,Xiao-Xiao Xie,Qi-Peng Shu,Xue-Hua Du,Lin-Zhi Qi,Xiao-Dong Zhang,Ming-Hui Zhang,Guang Shan,Run-Lei Du,Shang-Ze Li
View all publications
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