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AB183706

Anti-NDUFA5 antibody [EPR15330]

2

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal NDUFA5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, Complex I subunit B13, Complex I-13kD-B, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, CI-13kD-B, NDUFA5

2 Images
Western blot - Anti-NDUFA5 antibody [EPR15330] (AB183706)
  • WB

Supplier Data

Western blot - Anti-NDUFA5 antibody [EPR15330] (AB183706)

All lanes:

Western blot - Anti-NDUFA5 antibody [EPR15330] (ab183706) at 1/20000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Western blot - Anti-NDUFA5 antibody [EPR15330] (AB183706)
  • WB

Supplier Data

Western blot - Anti-NDUFA5 antibody [EPR15330] (AB183706)

All lanes:

Western blot - Anti-NDUFA5 antibody [EPR15330] (ab183706) at 1/5000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HT-1376 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

  • Carrier free

    Anti-NDUFA5 antibody [EPR15330] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15330

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA5 also known as NADH:Ubiquinone Oxidoreductase Subunit A5 is part of the mitochondrial respiratory chain complex I. This protein has an approximate molecular mass of 15 kDa. NDUFA5 is expressed in many tissues but shows higher levels in energy-demanding organs such as the heart brain and skeletal muscle. It contributes to cellular respiration by assisting in electron transfer from NADH to ubiquinone which is critical in energy production.
Biological function summary

NDUFA5 is an essential component of Complex I in the mitochondrial inner membrane. Complex I the largest enzyme complex of the mitochondrial electron transport chain consists of 45 subunits. NDUFA5 functions by aiding electron transfer through this complex. Proper function of NDUFA5 is vital for ATP generation through oxidative phosphorylation an important process in bioenergetics.

Pathways

The role of NDUFA5 in the electron transport chain places it central to the oxidative phosphorylation pathway. This pathway also includes other complexes such as Complex III and IV which continue electron transfer leading to ATP synthesis. NDUFA5 interacts closely with other subunits within Complex I like NDUFS2 and forms a luminal proton gradient necessary for ATP production.

NDUFA5 mutations have links to mitochondrial Complex I deficiency a condition causing disorders in energy metabolism. This deficiency can lead to a wide range of clinical manifestations including Leigh syndrome a severe neurological disorder. NDUFA5 dysfunction affects energy-intensive tissues and its abnormal activity could involve other proteins in Complex I such as NDUFS4 which together contribute to the pathology of these mitochondrial disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFA5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 289:14719-30 PubMed24713701

2014

Altering O-linked β-N-acetylglucosamine cycling disrupts mitochondrial function.

Applications

WB

Species

Unspecified reactive species

Ee Phie Tan,Maria T Villar,Lezi E,Jianghua Lu,J Eva Selfridge,Antonio Artigues,Russell H Swerdlow,Chad Slawson
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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