JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250683

Anti-NDUFA5 antibody [EPR15330] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal NDUFA5 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, Complex I subunit B13, Complex I-13kD-B, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, CI-13kD-B, NDUFA5

2 Images
Western blot - Anti-NDUFA5 antibody [EPR15330] - BSA and Azide free (AB250683)
  • WB

Supplier Data

Western blot - Anti-NDUFA5 antibody [EPR15330] - BSA and Azide free (AB250683)

This data was developed using ab183706, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFA5 antibody [EPR15330] (<a href='/en-us/products/primary-antibodies/ndufa5-antibody-epr15330-ab183706'>ab183706</a>) at 1/20000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Western blot - Anti-NDUFA5 antibody [EPR15330] - BSA and Azide free (AB250683)
  • WB

Supplier Data

Western blot - Anti-NDUFA5 antibody [EPR15330] - BSA and Azide free (AB250683)

This data was developed using ab183706, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFA5 antibody [EPR15330] (<a href='/en-us/products/primary-antibodies/ndufa5-antibody-epr15330-ab183706'>ab183706</a>) at 1/5000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HT-1376 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15330

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250683 is the carrier-free version of ab183706.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA5 also known as NADH:Ubiquinone Oxidoreductase Subunit A5 is part of the mitochondrial respiratory chain complex I. This protein has an approximate molecular mass of 15 kDa. NDUFA5 is expressed in many tissues but shows higher levels in energy-demanding organs such as the heart brain and skeletal muscle. It contributes to cellular respiration by assisting in electron transfer from NADH to ubiquinone which is critical in energy production.
Biological function summary

NDUFA5 is an essential component of Complex I in the mitochondrial inner membrane. Complex I the largest enzyme complex of the mitochondrial electron transport chain consists of 45 subunits. NDUFA5 functions by aiding electron transfer through this complex. Proper function of NDUFA5 is vital for ATP generation through oxidative phosphorylation an important process in bioenergetics.

Pathways

The role of NDUFA5 in the electron transport chain places it central to the oxidative phosphorylation pathway. This pathway also includes other complexes such as Complex III and IV which continue electron transfer leading to ATP synthesis. NDUFA5 interacts closely with other subunits within Complex I like NDUFS2 and forms a luminal proton gradient necessary for ATP production.

NDUFA5 mutations have links to mitochondrial Complex I deficiency a condition causing disorders in energy metabolism. This deficiency can lead to a wide range of clinical manifestations including Leigh syndrome a severe neurological disorder. NDUFA5 dysfunction affects energy-intensive tissues and its abnormal activity could involve other proteins in Complex I such as NDUFS4 which together contribute to the pathology of these mitochondrial disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFA5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed31094704

2019

Protective role of B cells in sterile particulate-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaikh M Atif,Douglas G Mack,Amy S McKee,Javier Rangel-Moreno,Allison K Martin,Andrew Getahun,Lisa A Maier,John C Cambier,Rubin Tuder,Andrew P Fontenot
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB198196

Anti-NDUFA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA2 antibody (AB198196)

  • 0
  • 0
Primary Antibodies

AB200198

Anti-NDUFB9 antibody [EPR15955-78]

RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955-78] (AB200198)

  • 0
  • 0
Primary Antibodies

AB110258

Anti-MTCO2 antibody [12C4F12]

Western blot - Anti-MTCO2 antibody [12C4F12] (AB110258)

  • 5
  • 18
Primary Antibodies

AB14713

Anti-NDUFA9 antibody [20C11B11B11]

KO Validated

Western blot - Anti-NDUFA9 antibody [20C11B11B11] (AB14713)

  • 4
  • 8

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com