AB140871

Anti-NDUFA7 antibody

Name: Anti-NDUFA7 antibody (ab140871) | Abcam
Brand: Abcam Limited
SKU: AB140871

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(2 Publications)

Goat Polyclonal NDUFA7 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 aa 1-100.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, Complex I-B14.5a, NADH-ubiquinone oxidoreductase subunit B14.5a, CI-B14.5a, NDUFA7

2 Images

Supplier Data

Western blot - Anti-NDUFA7 antibody (AB140871)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-NDUFA7 antibody (ab140871) at 0.1 µg/mL

Lane 1:

Human heart lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse heart lysate (in RIPA buffer) at 35 µg

Lane 3:

Rat heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 13 kDa

Observed band size: 15 kDa

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Supplier Data

Western blot - Anti-NDUFA7 antibody (AB140871)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-NDUFA7 antibody (ab140871) at 0.1 µg/mL

All lanes:

Pig heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 13 kDa

Observed band size: 15 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O95182

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-8 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NDUFA7 protein also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 is an integral part of the mitochondrial respiratory chain. It has a molecular weight of approximately 12 kDa. This protein is expressed mostly in the mitochondria of various cells and tissues such as muscle liver and brain. It plays a significant role in the electron transport chain where it contributes to the transfer of electrons from NADH to ubiquinone therefore contributing to ATP production.

Biological function summary

The NDUFA7 protein operates as a component of the mitochondrial complex I also referred to as NADH:ubiquinone oxidoreductase. This complex which includes around 45 different subunits is the first and largest enzyme of the mitochondrial respiratory chain. NDUFA7 does not directly catalyze the electron transfer but stabilizes the structure of the complex necessary for efficient electron flow. Proper functioning of complex I is essential for maintaining cellular energy homeostasis and normal metabolic activity.

Pathways

NDUFA7 plays an important role within the oxidative phosphorylation pathway which is important for aerobic energy production. This protein is closely related to other proteins in complex I such as NDUFA9 which together ensure efficient functioning of the electron transfer process. Moreover NDUFA7 indirectly influences other metabolic networks that rely on ATP including the TCA cycle and fatty acid oxidation highlighting its fundamental contribution to cellular metabolism.

Mutations in the NDUFA7 gene can lead to mitochondrial disorders such as Leigh syndrome and mitochondrial complex I deficiency. These are serious conditions that may result in motor disability developmental challenges and other systemic problems. In the context of these disorders NDUFA7 interacts with proteins like NDUFS1 which also constitutes a part of complex I and defects in these proteins can amplify mitochondrial dysfunction highlighting the interconnectedness of protein actions within the cell.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine : PubMed32989924

2020

ndufa7 plays a critical role in cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Xingjuan Shi,Yu Zhang,Ru Chen,Yijie Gong,Mingming Zhang,Rui Guan,Ori D Rotstein,Xiangdong Liu,Xiao-Yan Wen

Frontiers in physiology 10:252 PubMed30914972

2019

Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Zhendong Zhu,Takashi Umehara,Tetsuji Okazaki,Masaaki Goto,Yoko Fujita,S A Masudul Hoque,Tomoko Kawai,Wenxian Zeng,Masayuki Shimada

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFA7 antibody

Western blot - Anti-NDUFA7 antibody (AB140871)

Western blot - Anti-NDUFA7 antibody (AB140871)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise