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AB184952

Anti-NDUFA8 antibody [EPR15303]

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(7 Publications)

Rabbit Recombinant Monoclonal NDUFA8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, Complex I-19kD, Complex I-PGIV, NADH-ubiquinone oxidoreductase 19 kDa subunit, CI-19kD, CI-PGIV, NDUFA8

2 Images
Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (AB184952)
  • IP

Supplier Data

Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (AB184952)

Western blot analysis of NDUFA8 in Human fetal heart lysate immunoprecipitated with ab184952 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Human fetal heart lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (ab184952)

Predicted band size: 20 kDa

Observed band size: 17 kDa

false

Western blot - Anti-NDUFA8 antibody [EPR15303] (AB184952)
  • WB

Supplier Data

Western blot - Anti-NDUFA8 antibody [EPR15303] (AB184952)

All lanes:

Western blot - Anti-NDUFA8 antibody [EPR15303] (ab184952) at 1/20000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human fetal muscle lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 17 kDa

false

  • Carrier free

    Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15303

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA8 also known as the NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 is a protein with a mass of approximately 23 kDa. Mechanically it is an integral component of the mitochondrial respiratory chain specifically part of Complex I. This protein is expressed in multiple tissues with high levels in energy-demanding organs such as the heart brain and muscles. NDUFA8 plays a role in the catalytic activity of Complex I contributing to electron transfer and assisting in the creation of the proton gradient essential for ATP production.
Biological function summary

NDUFA8 participates in energy metabolism by facilitating the initial steps of the oxidative phosphorylation pathway. It is a subunit of Complex I also known as NADH:ubiquinone oxidoreductase one of the largest components in the mitochondrial inner membrane. This complex is important for the transfer of electrons from NADH to ubiquinone contributing to a process that generates ATP. NDUFA8 through its interaction within this complex helps regulate mitochondrial function and cellular energy balance.

Pathways

Energy metabolism involves the participation of NDUFA8 in the oxidative phosphorylation and mitochondrial electron transport chain pathways. These pathways are important for energy production in the form of ATP synthesis. NDUFA8 operates closely with other Complex I subunits and works in tandem with related complexes like Complexes II III and IV. Proteins such as NDUFA1 and NDUFB5 are important partners within these pathways ensuring the efficient execution of electron transport and ATP generation.

Defects in NDUFA8 have associations with mitochondrial disorders and neurodegenerative diseases. Mutations or dysfunctions in NDUFA8 can contribute to conditions such as Leigh syndrome and Complex I deficiency. These disorders involve impaired mitochondrial function leading to neurodegenerative symptoms and energy production deficits. Proteins connected to these diseases including other Complex I components like NDUFS4 exhibit interactions that highlight their critical roles in maintaining cellular energy homeostasis and neurological function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis (PubMed : 27626371, PubMed : 32385911, PubMed : 33153867). Complex I functions in the transfer of electrons from NADH to the respiratory chain (PubMed : 27626371). The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed : 27626371).
See full target information NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5133 PubMed40461459

2025

ROMO1 overexpression protects the mitochondrial cysteinome from oxidations in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Fengli Xu,Haipeng Huang,Kun Peng,Chongshu Jian,Hao Wu,Zhiwen Jing,Shan Qiu,Ying Chen,Keke Liu,Ling Fu,Yanru Wang,Jing Yang,Xiaotao Duan,Chu Wang,Heping Cheng,Xianhua Wang

Science advances 10:eado5887 PubMed39454000

2024

Mitochondrial fatty acid oxidation drives senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Shota Yamauchi,Yuki Sugiura,Junji Yamaguchi,Xiangyu Zhou,Satoshi Takenaka,Takeru Odawara,Shunsuke Fukaya,Takao Fujisawa,Isao Naguro,Yasuo Uchiyama,Akiko Takahashi,Hidenori Ichijo

RNA (New York, N.Y.) 30:223-239 PubMed38164626

2024

LARP4 is an RNA-binding protein that binds nuclear-encoded mitochondrial mRNAs to promote mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin M Lewis,Chae Yun Cho,Hsuan-Lin Her,Orel Mizrahi,Tony Hunter,Gene W Yeo

Antioxidants (Basel, Switzerland) 10: PubMed34943057

2021

Mito-TIPTP Increases Mitochondrial Function by Repressing the Rubicon-p22phox Interaction in Colitis-Induced Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Sung Kim,Ye-Ram Kim,Sein Jang,Sang Geon Wang,Euni Cho,Seok-Jun Mun,Hye-In Jeon,Hyo-Keun Kim,Sun-Joon Min,Chul-Su Yang

Frontiers in physiology 12:649461 PubMed33897458

2021

Altered Transcription Factor Expression Responses to Exercise in Insulin Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Rocio Zapata-Bustos,Jean Finlayson,Paul R Langlais,Dawn K Coletta,Moulun Luo,Danielle Grandjean,Elena A De Filippis,Lawrence Mandarino

Clinical genetics 98:155-165 PubMed32385911

2020

A homozygous variant in NDUFA8 is associated with developmental delay, microcephaly, and epilepsy due to mitochondrial complex I deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yukiko Yatsuka,Yoshihito Kishita,Luke E Formosa,Masaru Shimura,Fumihito Nozaki,Tatsuya Fujii,Kazuhiro R Nitta,Akira Ohtake,Kei Murayama,Michael T Ryan,Yasushi Okazaki

Cell reports 26:759-774.e5 PubMed30650365

2019

Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Habich,Silja Lucia Salscheider,Lena Maria Murschall,Michaela Nicole Hoehne,Manuel Fischer,Fabian Schorn,Carmelina Petrungaro,Muna Ali,Alican J Erdogan,Shadi Abou-Eid,Hamid Kashkar,Joern Dengjel,Jan Riemer
View all publications
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Product promise

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