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AB250789

Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NDUFA8 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, Complex I-19kD, Complex I-PGIV, NADH-ubiquinone oxidoreductase 19 kDa subunit, CI-19kD, CI-PGIV, NDUFA8

2 Images
Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (AB250789)
  • IP

Supplier Data

Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (AB250789)

This data was developed using ab184952, the same antibody clone in a different buffer formulation.

Western blot analysis of NDUFA8 in Human fetal heart lysate immunoprecipitated with ab184952 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Human fetal heart lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (<a href='/en-us/products/primary-antibodies/ndufa8-antibody-epr15303-ab184952'>ab184952</a>)

Predicted band size: 20 kDa

Observed band size: 17 kDa

false

Western blot - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (AB250789)
  • WB

Supplier Data

Western blot - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (AB250789)

This data was developed using ab184952, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFA8 antibody [EPR15303] (<a href='/en-us/products/primary-antibodies/ndufa8-antibody-epr15303-ab184952'>ab184952</a>) at 1/20000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human fetal muscle lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15303

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250789 is the carrier-free version of ab184952.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFA8 also known as the NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 is a protein with a mass of approximately 23 kDa. Mechanically it is an integral component of the mitochondrial respiratory chain specifically part of Complex I. This protein is expressed in multiple tissues with high levels in energy-demanding organs such as the heart brain and muscles. NDUFA8 plays a role in the catalytic activity of Complex I contributing to electron transfer and assisting in the creation of the proton gradient essential for ATP production.
Biological function summary

NDUFA8 participates in energy metabolism by facilitating the initial steps of the oxidative phosphorylation pathway. It is a subunit of Complex I also known as NADH:ubiquinone oxidoreductase one of the largest components in the mitochondrial inner membrane. This complex is important for the transfer of electrons from NADH to ubiquinone contributing to a process that generates ATP. NDUFA8 through its interaction within this complex helps regulate mitochondrial function and cellular energy balance.

Pathways

Energy metabolism involves the participation of NDUFA8 in the oxidative phosphorylation and mitochondrial electron transport chain pathways. These pathways are important for energy production in the form of ATP synthesis. NDUFA8 operates closely with other Complex I subunits and works in tandem with related complexes like Complexes II III and IV. Proteins such as NDUFA1 and NDUFB5 are important partners within these pathways ensuring the efficient execution of electron transport and ATP generation.

Defects in NDUFA8 have associations with mitochondrial disorders and neurodegenerative diseases. Mutations or dysfunctions in NDUFA8 can contribute to conditions such as Leigh syndrome and Complex I deficiency. These disorders involve impaired mitochondrial function leading to neurodegenerative symptoms and energy production deficits. Proteins connected to these diseases including other Complex I components like NDUFS4 exhibit interactions that highlight their critical roles in maintaining cellular energy homeostasis and neurological function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis (PubMed : 27626371, PubMed : 32385911, PubMed : 33153867). Complex I functions in the transfer of electrons from NADH to the respiratory chain (PubMed : 27626371). The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed : 27626371).
See full target information NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2021:5523726 PubMed34422071

2021

Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Wei Liu,Yong-Li Wu,Wei Wei,Yan-Ling Zhang,Di Liu,Xiao-Xiu Ma,Chun Li,Yu-Yuan Ma
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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