Mouse Monoclonal NDUFA9 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat, Cow samples. Cited in 246 publications.
IgG1
Mouse
pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Liquid
Monoclonal
Flow Cyt | WB | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Cow | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Required for proper complex I assembly (PubMed:28671271). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
NDUFS2L, NDUFA9, NDUFS2L, Complex I-39kD, NADH-ubiquinone oxidoreductase 39 kDa subunit, CI-39kD
Mouse Monoclonal NDUFA9 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat, Cow samples. Cited in 246 publications.
IgG1
Mouse
pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Liquid
Monoclonal
20C11B11B11
IgG fraction
kappa
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Blue Ice
+4°C
+4°C
This monoclonal antibody to NDUFA9 has been knockout validated in Western blot. The expected band for NDUFA9 was observed in wild type cells and the band was not seen in knockout cells.
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This supplementary information is collated from multiple sources and compiled automatically.
The NDUFA9 also known as NADH: ubiquinone oxidoreductase subunit A9 plays a mechanical role as a core component of Complex I in the mitochondrial respiratory chain. This protein facilitates the transfer of electrons from NADH to ubiquinone an essential step in cellular energy production. NDUFA9 has a molecular weight of about 39 kDa and is expressed in tissues with high energy demands like the heart and brain.
NDUFA9 functions as an important element of the mitochondrial respiratory complex I contributing to ATP production through oxidative phosphorylation. It is part of the multi-subunit enzyme Complex I which consists of other integral subunits such as NDUFS1 and NDUFV2. These elements work synergistically for efficient electron transport and proton pumping across the mitochondrial inner membrane.
NDUFA9 integrates into essential pathways like the electron transport chain and oxidative phosphorylation pathway. It works closely with proteins such as NDUFS3 and NDUFB8 essential for the integrity and activity of Complex I. The electron transport chain plays a significant role in generating the proton-motive force necessary for ATP synthesis directly linking NDUFA9 to energy metabolism.
Dysfunction of NDUFA9 relates to mitochondrial disorders like Leigh syndrome and Parkinson's disease. Mutations or deficiencies in the NDUFA9 protein can impair electron transport and ATP production leading to cellular energy deficits. In Leigh syndrome other proteins such as ND3 and COX1 may be involved while in Parkinson's disease interactions with proteins like alpha-synuclein and PINK1 have been reported.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Overlay histogram showing HepG2 cells stained with ab14713 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14713, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: NDUFA9 knockout HAP1 cell lysate (20 μg)
Lane 3: WI38 cell lysate (20 μg)
Lane 4: NIH3T3 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab14713 observed at 40 kDa. Red - loading control, Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560, observed at 52 kDa.
ab14713 was shown to specifically react with NDUFA9 in wild-type HAP1 cells. No band was observed when NDUFA9 knockout HAP1 samples were used. Wild-type and NDUFA9 knockout samples were subjected to SDS-PAGE. ab14713 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (loading control to alpha tubulin) were diluted at 1μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)
Predicted band size: 43 kDa
ab14713 a 1/100 dilution staining NDUFA9 in Human spinal column tissue by Immunohistochemistry (Formalin/PFA-Fixed paraffin-embedded sections). Antibody was incubated with the sample for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse secondary (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temperature. Sections were washed x3 in PBST and peroxidase activity was demonstrated using kit. Antigen retrieval was performed by 1 minute of pressure cooking with 1 mmol EDTA pH 8.0.
The band observed at 36 kDa could potentially be a cleaved form of NDUFA9 due to the presence of a 35 amino acid transit peptide.
All lanes: Western blot - Anti-NDUFA9 antibody [20C11B11B11] (ab14713) at 1 µg/mL
Lane 1: WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 2: Human testis tissue lysate - total protein (ab30257) at 10 µg
Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 36 kDa, 58 kDa
Exposure time: 20min
All lanes: Western blot - Anti-NDUFA9 antibody [20C11B11B11] (ab14713)
Lane 1: Isolated mitochondria from Human heart at 5 µg
Lane 2: Isolated mitochondria from Bovine heart at 1 µg
Lane 3: Isolated mitochondria from Rat heart at 10 µg
Lane 4: Isolated mitochondria from Mouse Heart at 10 µg
All lanes: Goat anti-Mouse IgG
Predicted band size: 43 kDa
Observed band size: 37 kDa
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