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AB247556

Anti-NDUFAF1 antibody [EPR2795] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NDUFAF1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CIA30, CGI-65, NDUFAF1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2795] - BSA and Azide free (AB247556)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2795] - BSA and Azide free (AB247556)

This data was developed using ab92454, the same antibody clone in a different buffer formulation.

Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2795] - BSA and Azide free (AB247556)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2795] - BSA and Azide free (AB247556)

This data was developed using ab92454, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution.

Western blot - Anti-NDUFAF1 antibody [EPR2795] - BSA and Azide free (AB247556)
  • WB

Unknown

Western blot - Anti-NDUFAF1 antibody [EPR2795] - BSA and Azide free (AB247556)

This data was developed using ab92454, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFAF1 antibody [EPR2795] (<a href='/en-us/products/primary-antibodies/ndufaf1-antibody-epr2795-ab92454'>ab92454</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2795

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab247556 is the carrier-free version of ab92454.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFAF1 also known as NADH dehydrogenase (ubiquinone) complex I assembly factor 1 plays an essential role in the mitochondrial respiratory chain by assisting the assembly of complex I. This protein has a mass of approximately 36 kDa. NDUFAF1 expression occurs mainly in tissues with high metabolic activity like the brain heart and skeletal muscle. It facilitates the proper formation and function of the complex I assembly allowing efficient electron transport essential for cellular respiration.
Biological function summary

NDUFAF1 is a critical assembly factor integral to forming the mitochondrial complex I also known as NADH:ubiquinone oxidoreductase. NDUFAF1 contributes to the stability and proper function of this multi-component enzyme system which is important in cellular energy production. As part of the complex it ensures efficient ATP generation through oxidative phosphorylation which is the primary source of energy in aerobic eukaryotic cells.

Pathways

NDUFAF1 functions predominantly in the oxidative phosphorylation pathway. This involvement connects NDUFAF1 to the fundamental molecular processes sustaining life. The protein plays a role alongside others like NDUFV1 and NDUFS1 in maintaining complex I activity. These relationships ensure the effective transfer of electrons from NADH to ubiquinone promoting efficient energy production throughout the cell.

NDUFAF1 mutations or dysfunction have been linked to mitochondrial disorders such as Leigh syndrome and complex I deficiency. These conditions are often characterized by a disruption in mitochondrial energy metabolism leading to severe neurological and muscular symptoms. Furthermore NDUFAF1 dysfunction can indirectly affect other proteins like NDUFS4 which is also involved in complex I assembly and function exacerbating the disease phenotypes. Mitochondrial diseases often arise from impaired pathways involving several network proteins highlighting the significance of proper NDUFAF1 function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
See full target information NDUFAF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 30:666-679 PubMed35170865

2022

Exosomal miR-122 promotes adipogenesis and aggravates obesity through the VDR/SREBF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Huang,Ji-Xiong Chen,Yi Ren,Li-Chun Fan,Wei Xiang,Xiao-Jie He
View all publications
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Product promise

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