AB191414

Anti-NDUFAF4 antibody [EPR15931]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NDUFAF4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C6orf66, HRPAP20, HSPC125, My013, NDUFAF4, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, Hormone-regulated proliferation-associated protein of 20 kDa

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

Immunofluorescence analysis of HeLa cells labeling NDUFAF4 using ab191414 at 1/150 dilution (9.5 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain : DAPI. The 2 negative cotrols images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1 : 400 dilution as secondary antibody.

Supplier Data

Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

Immunoprecipitation analysis of MDA-MB-435 cell lysate labeling NDUFAF4 using ab191414 at 1/90 dilution (Lane 1). An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of MDA-MB-435 cell lysate.

All lanes:

Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] (ab191414)

Predicted band size: 20 kDa

false

Supplier Data

Western blot - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

All lanes:

Western blot - Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/10000 dilution

Lane 1:

T47-D cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

MDA-MB-435 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Supplier Data

Western blot - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

All lanes:

Western blot - Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/1000 dilution

All lanes:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Carrier free

Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15931

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/90", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFAF4 also known as Complex I Assembly Factor 2 plays an integral role in the assembly of NADH:ubiquinone oxidoreductase complex I within mitochondria. It has a mass of approximately 20 kilodaltons. This protein is widely expressed in various tissues with heightened levels found in organs with high energy demands such as the brain and heart. Functionally NDUFAF4 mediates the proper formation and stabilization of the mitochondrial complex I by contributing to the assembly of its subunits.

Biological function summary

NDUFAF4 participates in the mitochondrial electron transport chain as part of the complex I assembly. It acts as an assembly factor for complex I the first component of the chain which is important for initiating electron transport from NADH to the rest of the chain. This complex contains multiple subunits that require precise assembly and NDUFAF4 ensures the correct incorporation of these subunits. Its role is essential for maintaining efficient mitochondrial respiration thereby supporting ATP production.

Pathways

The NDUFAF4 protein ensures proper electron transport chain function by facilitating complex I assembly. It interacts within mitochondrial pathways involving oxidative phosphorylation and reactive oxygen species (ROS) regulation. It associates with other proteins such as NDUFS3 and NDUFS4 as part of the complex I formation process. Progressive assembly of complex I facilitated by NDUFAF4 is an important step in maintaining cellular energy metabolism.

Improper function or expression of NDUFAF4 leads to mitochondrial disorders including Leigh syndrome and other metabolic encephalopathies. Mutations in NDUFAF4 disrupt complex I assembly resulting in impaired mitochondrial respiration and energy production. This protein also relates to Parkinson’s disease where its involvement in complex I assembly connects it to proteins like Parkin which are associated with mitochondrial dysfunction in dopaminergic neurons.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the assembly of mitochondrial NADH : ubiquinone oxidoreductase complex (complex I) (PubMed : 18179882, PubMed : 28853723). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.

See full target information NDUFAF4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular carcinogenesis 63:145-159 PubMed37787384

2023

A genetic variant in gene NDUFAF4 confers the risk of non-small cell lung cancer by perturbing hsa-miR-215 binding.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Long,Jiaoyuan Li,Tongxin Yin,Ke Liu,Yi Wang,Jieyi Long,Jianing Wang,Liming Cheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFAF4 antibody [EPR15931]

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

Western blot - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

Western blot - Anti-NDUFAF4 antibody [EPR15931] (AB191414)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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