Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (AB251070)

This data was developed using ab191414, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling NDUFAF4 using ab191414 at 1/150 dilution (9.5 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain : DAPI. The 2 negative cotrols images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1 : 400 dilution as secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (AB251070)

This data was developed using ab191414, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of MDA-MB-435 cell lysate labeling NDUFAF4 using ab191414 at 1/90 dilution (Lane 1). An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of MDA-MB-435 cell lysate.

All lanes:

Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] (<a href='/en-us/products/primary-antibodies/ndufaf4-antibody-epr15931-ab191414'>ab191414</a>)

Predicted band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (AB251070)

This data was developed using ab191414, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFAF4 antibody [EPR15931] (<a href='/en-us/products/primary-antibodies/ndufaf4-antibody-epr15931-ab191414'>ab191414</a>) at 1/10000 dilution

Lane 1:

T47-D cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

MDA-MB-435 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (AB251070)

This data was developed using ab191414, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFAF4 antibody [EPR15931] (<a href='/en-us/products/primary-antibodies/ndufaf4-antibody-epr15931-ab191414'>ab191414</a>) at 1/1000 dilution

All lanes:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false