Rabbit Recombinant Monoclonal NDUFAF5 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 3 publications.
Images
![Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--western-blot-img58498.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235)")
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--immunocytochemistry-immunofluorescence-img70398.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235)")
![Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--western-blot-img61460.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235)")
![Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--western-blot-img63044.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (AB192235)")
Publications
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- Journal of Cancer 12:860-8732021Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLidong Wang et. al.PubMed 33403043
- American journal of human genetics 106:92-1012019Pathogenic Bi-allelic Mutations in NDUFAF8 Cause Leigh Syndrome with an Isolated Complex I Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte L Alston,Mike T Veling,Juliana Heidler,Lucie S Taylor,Joseph T Alaimo,Andrew Y Sung,Langping He,Sila Hopton,Alexander Broomfield,Julija Pavaine,Jullianne Diaz,Eyby Leon,Philipp Wolf,Robert McFarland,Holger Prokisch,Saskia B Wortmann,Penelope E Bonnen,Ilka Wittig,David J Pagliarini,Robert W TaylorPubMed 31866046
- Molecular genetics and metabolism 126:53-632018Novel mutations in the mitochondrial complex I assembly gene NDUFAF5 reveal heterogeneous phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariella T Simon,Shaya S Eftekharian,Alexander E Stover,Aaron F Osborne,Bruce H Braffman,Richard C Chang,Raymond Y Wang,Maija R Steenari,Sha Tang,Paul Wuh-Liang Hwu,Ryan J Taft,Paul J Benke,Jose E AbdenurPubMed 30473481
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected | Tested |
| Mouse | Expected | Tested | Tested | Tested | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/400 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Arginine hydroxylase involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages (PubMed:18940309, PubMed:27226634). Acts by mediating hydroxylation of 'Arg-111' of NDUFS7 (PubMed:27226634). May also have methyltransferase activity (Probable).
Alternative names
C20orf7, NDUFAF5, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5, Putative methyltransferase NDUFAF5
Recommended products
Rabbit Recombinant Monoclonal NDUFAF5 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15870
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NDUFAF5 also known as NADH:ubiquinone oxidoreductase complex assembly factor 5 is a mitochondrial protein with a mass of approximately 34 kDa. The protein plays a mechanical role in the assembly of the mitochondrial complex I also termed NADH dehydrogenase. It's necessary for the assembly of this critical component of the electron transport chain which is fundamental for cellular energy production. NDUFAF5 is expressed in various tissues with significant expression in energy-demanding organs such as the heart and brain.
- Biological function summary
NDUFAF5 participates in the assembly of complex I an essential part of mitochondrial oxidative phosphorylation. This complex is a multi-subunit structure where NDUFAF5 functions as an important assembly factor interacting with precursor subunits to help build the complex properly. It coordinates the junction of early-stage assembly intermediates working closely with assembly proteins like NDUFAF1 to ensure the formation and maintenance of efficient electron transport.
- Pathways
NDUFAF5 is an important player in the mitochondrial oxidative phosphorylation pathway and the electron transport chain. These pathways are responsible for ATP production through the transfer of electrons from NADH to oxygen. NDUFAF5 ensures that complex I integrates correctly into the pathway assisting with proper electron and proton flow. Mitochondrial proteins such as NDUFS1 and CYCS interact closely with NDUFAF5 within these pathways contributing to normal cellular respiration and energy metabolism.
- Associated diseases and disorders
Dysfunctions in NDUFAF5 are linked with complex I deficiency and mitochondrial disorders such as Leigh syndrome. These conditions can result in severe energy production impairments leading to neurological symptoms and muscle weakness. Mutations in NDUFAF5 can alter interactions with assembly proteins such as NDUFAF1 affecting the stability and function of the entire complex I. Observing the involvement of NDUFAF5 could provide insights into the pathological mechanisms of mitochondrial-related diseases and aid in therapeutic development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--western-blot-img58498.jpg "Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
All lanes: Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235) at 1/5000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: 293 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137962.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--immunocytochemistry-immunofluorescence-img70398.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
Immunofluorescent analysis of NIH 3T3 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFAF5 with ab192235 at 1/400 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-NDUFAF5 at 1/400 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.![Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--western-blot-img61460.jpg "Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
All lanes: Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate at 10 µg
Lane 2: Human fetal liver lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--western-blot-img63044.jpg "Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
All lanes: Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Mouse spleen lysate at 10 µg
Lane 5: RAW 264.7 lysate at 10 µg
Lane 6: NIH 3T3 lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--immunoprecipitation-img89821.jpg "Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
Western blot analysis of pellet from 293 cell lysate immunoprecipitated using ab192235 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.All lanes: Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138814.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--flow-cytometry-intracellular-img13661.jpg "Flow Cytometry (Intracellular) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
Flow Cytometry (Intracellular) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NDUFAF5 (red) with purified ab192235 at dilution of 1/200. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
![Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--immunoprecipitation-img89821.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
![Flow Cytometry (Intracellular) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235), expandable thumbnail](https://content.abcam.com/products/images/ndufaf5-antibody-epr15870-c-terminal-ab192235--flow-cytometry-intracellular-img13661.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)")
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