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AB201302

Anti-NDUFB1 antibody

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(4 Publications)

Rabbit Polyclonal NDUFB1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NDUFB1 aa 1 to C-terminus.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, Complex I-MNLL, NADH-ubiquinone oxidoreductase MNLL subunit, CI-MNLL, NDUFB1

1 Images
Western blot - Anti-NDUFB1 antibody (AB201302)
  • WB

Supplier Data

Western blot - Anti-NDUFB1 antibody (AB201302)

All lanes:

Western blot - Anti-NDUFB1 antibody (ab201302)

Lane 1:

HEK293T whole cell lysate

Lane 2:

mouse RAW 264.7 whole cell lysate

Lane 3:

rat H9C2 whole cell lysate

Lane 4:

HeLa whole cell lysate

Predicted band size: 7 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NDUFB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75438

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab201302 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFB1 also known as NADH:ubiquinone oxidoreductase subunit B1 is a component of complex I in the mitochondrial respiratory chain. It plays a role in the transfer of electrons from NADH to ubiquinone. NDUFB1 has a molecular mass of approximately 12.7 kDa. Expression of NDUFB1 is observed in high energy-demanding tissues like the heart brain and skeletal muscles.
Biological function summary

NDUFB1 functions as part of the mitochondrial complex I which is the first enzyme of the electron transport chain. Complex I is essential for energy production in cells as it facilitates the transfer of electrons and contributes to proton gradient formation across the inner mitochondrial membrane. NDUFB1 interacts with other subunits within complex I to ensure efficient electron flow impacting the overall respiratory activity and ATP synthesis.

Pathways

NDUFB1 is involved with the oxidative phosphorylation pathway where it collaborates with other components of complex I like NDUFS7 and NDUFV1. It also associates with the mitochondrial biogenesis pathway important for maintaining mitochondrial function and energy metabolism. Through these pathways NDUFB1 helps to sustain cellular energy balance and integrate mitochondrial signaling processes.

Defects in NDUFB1 have been connected to conditions such as mitochondrial complex I deficiency and Leigh syndrome. These diseases result from impaired energy metabolism leading to severe neurological and muscular symptoms. NDUFB1 dysfunction can influence the activity of proteins like NDUFA1 and NDUFS1 amplifying mitochondrial dysfunction and contributing to the progression of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFB1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular research 119:2213-2229 PubMed37395010

2023

Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSIT.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Nicol,Sara Falcone,Andrew Blease,Pratik Vikhe,Gabriele Civiletto,Saleh Salman Omairi,Carlo Viscomi,Ketan Patel,Paul K Potter

Life (Basel, Switzerland) 12: PubMed36013387

2022

Tunicamycin-Induced Endoplasmic Reticulum Stress Damages Complex I in Cardiac Mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Chen,Jeremy Thompson,Ying Hu,Edward J Lesnefsky

iScience 25:104193 PubMed35479410

2022

EMT alterations in the solute carrier landscape uncover SLC22A10/A15 imposed vulnerabilities in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Debasis Nayak,Brenna Weadick,Avinash K Persaud,Radhika Raj,Reena Shakya,Junan Li,Moray J Campbell,Rajgopal Govindarajan

Archives of biochemistry and biophysics 683:108299 PubMed32061585

2020

Endoplasmic reticulum stress-induced complex I defect: Central role of calcium overload.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed A Mohsin,Jeremy Thompson,Ying Hu,John Hollander,Edward J Lesnefsky,Qun Chen
View all publications
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