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AB317412

Anti-NDUFB1 antibody [S04-4H3]

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Rabbit Monoclonal NDUFB1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NDUFB1.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, Complex I-MNLL, NADH-ubiquinone oxidoreductase MNLL subunit, CI-MNLL, NDUFB1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB1 Antibody [S04-4H3] (AB317412)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB1 Antibody [S04-4H3] (AB317412)

Immunohistochemistry of NDUFB1 in paraffin-embedded human colon cancer tissue using ab317412 at dilution 1/50.

Western blot - Anti-NDUFB1 Antibody [S04-4H3] (AB317412)
  • WB

Supplier Data

Western blot - Anti-NDUFB1 Antibody [S04-4H3] (AB317412)

All lanes:

Western blot - Anti-NDUFB1 Antibody [S04-4H3] (ab317412) at 1/1000 dilution

Lane 1:

A459 cell lysate

Lane 2:

HL-60 cell lysate

Lane 3:

U2OS cell lysate

Predicted band size: 7 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S04-4H3

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NDUFB1.

O75438

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Cell Lines & Lysates

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Cell Lines & Lysates

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFB1 also known as NADH:ubiquinone oxidoreductase subunit B1 is a component of complex I in the mitochondrial respiratory chain. It plays a role in the transfer of electrons from NADH to ubiquinone. NDUFB1 has a molecular mass of approximately 12.7 kDa. Expression of NDUFB1 is observed in high energy-demanding tissues like the heart brain and skeletal muscles.
Biological function summary

NDUFB1 functions as part of the mitochondrial complex I which is the first enzyme of the electron transport chain. Complex I is essential for energy production in cells as it facilitates the transfer of electrons and contributes to proton gradient formation across the inner mitochondrial membrane. NDUFB1 interacts with other subunits within complex I to ensure efficient electron flow impacting the overall respiratory activity and ATP synthesis.

Pathways

NDUFB1 is involved with the oxidative phosphorylation pathway where it collaborates with other components of complex I like NDUFS7 and NDUFV1. It also associates with the mitochondrial biogenesis pathway important for maintaining mitochondrial function and energy metabolism. Through these pathways NDUFB1 helps to sustain cellular energy balance and integrate mitochondrial signaling processes.

Defects in NDUFB1 have been connected to conditions such as mitochondrial complex I deficiency and Leigh syndrome. These diseases result from impaired energy metabolism leading to severe neurological and muscular symptoms. NDUFB1 dysfunction can influence the activity of proteins like NDUFA1 and NDUFS1 amplifying mitochondrial dysfunction and contributing to the progression of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFB1
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Product promise

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