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Rabbit Recombinant Monoclonal NDUFB10 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 10 publications.


Images

Western blot - Anti-NDUFB10 antibody [EPR16230-47] (AB196019), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (AB196019), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (AB196019), expandable thumbnail
  • Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (AB196019), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NDUFB10 antibody [EPR16230-47] (AB196019), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/350

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/800

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Accessory subunit that is involved in the functional assembly of the mitochondrial respiratory chain complex I. Complex I has an NADH dehydrogenase activity with ubiquinone as an immediate electron acceptor and mediates the transfer of electrons from NADH to the respiratory chain.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal NDUFB10 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 10 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16230-47

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The NDUFB10 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex 10 is a component of the mitochondrial respiratory chain complex I. This protein has an approximate mass of 20 kDa. It is expressed mainly in the mitochondria of eukaryotic cells where it plays an important role in the process of oxidative phosphorylation. NDUFB10 is integral to the inner mitochondrial membrane where it acts as part of the machinery that facilitates electron transfer from NADH to ubiquinone.

Biological function summary

NDUFB10 plays a significant role in cellular energy production by being a subunit of complex I in the mitochondrial electron transport chain. As part of this large enzyme complex NDUFB10 contributes to the overall stability and function of complex I facilitating ATP production by driving the conversion of energy derived from nutrients into a usable form. Its presence in the mitochondrial inner membrane highlights its essential role in maintaining efficient energy metabolism and cellular respiration.

Pathways

The electron transport chain pathway incorporates NDUFB10 as a vital component. This pathway plays a major role in oxidative phosphorylation where NDUFB10 interacts closely with other mitochondrial proteins like NDUFS1 and NDUFA1 within complex I. Besides serving in ATP synthesis these interactions help in maintaining overall cellular bioenergetics and proper mitochondrial function demonstrating the integrated role NDUFB10 plays alongside other proteins in energy metabolism.

Associated diseases and disorders

Malfunction or mutations in NDUFB10 have been linked to mitochondrial disorders including mitochondrial encephalomyopathy and Leber's hereditary optic neuropathy. These conditions often involve disrupted energy metabolism which is critical for tissues with high energy demands. Associated proteins like NDUFS2 and NDUFV1 which also contribute to the function of complex I can compound the severity of these disorders if mutated underlining the importance of NDUFB10 within the broader context of mitochondrial health and disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019), expandable thumbnail

    Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019) at 1/10000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NDUFB10 with ab196019 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.

    Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NDUFB10 with ab196019 at 1/350 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue).

  • Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019), expandable thumbnail

    Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    NDUFB10 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab196019 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab196019 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell extract 10 μg (Input). Lane 2: ab196019 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab196019 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Predicted band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB10 (red) with purified ab196019 at a dilution of 1/800. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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