AB192245

Anti-NDUFB10 antibody [EPR16230]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal NDUFB10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, Complex I-PDSW, NADH-ubiquinone oxidoreductase PDSW subunit, CI-PDSW, NDUFB10

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230] (AB192245)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230] (AB192245)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB10 with ab192245 at 1/50 dilution ( 6 μg/ml) followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

The two negative controls are ab192245 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

Supplier Data

Western blot - Anti-NDUFB10 antibody [EPR16230] (AB192245)

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-NDUFB10 antibody [EPR16230] (ab192245) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Carrier free

Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16230

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NDUFB10 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex 10 is a component of the mitochondrial respiratory chain complex I. This protein has an approximate mass of 20 kDa. It is expressed mainly in the mitochondria of eukaryotic cells where it plays an important role in the process of oxidative phosphorylation. NDUFB10 is integral to the inner mitochondrial membrane where it acts as part of the machinery that facilitates electron transfer from NADH to ubiquinone.

Biological function summary

NDUFB10 plays a significant role in cellular energy production by being a subunit of complex I in the mitochondrial electron transport chain. As part of this large enzyme complex NDUFB10 contributes to the overall stability and function of complex I facilitating ATP production by driving the conversion of energy derived from nutrients into a usable form. Its presence in the mitochondrial inner membrane highlights its essential role in maintaining efficient energy metabolism and cellular respiration.

Pathways

The electron transport chain pathway incorporates NDUFB10 as a vital component. This pathway plays a major role in oxidative phosphorylation where NDUFB10 interacts closely with other mitochondrial proteins like NDUFS1 and NDUFA1 within complex I. Besides serving in ATP synthesis these interactions help in maintaining overall cellular bioenergetics and proper mitochondrial function demonstrating the integrated role NDUFB10 plays alongside other proteins in energy metabolism.

Malfunction or mutations in NDUFB10 have been linked to mitochondrial disorders including mitochondrial encephalomyopathy and Leber's hereditary optic neuropathy. These conditions often involve disrupted energy metabolism which is critical for tissues with high energy demands. Associated proteins like NDUFS2 and NDUFV1 which also contribute to the function of complex I can compound the severity of these disorders if mutated underlining the importance of NDUFB10 within the broader context of mitochondrial health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit that is involved in the functional assembly of the mitochondrial respiratory chain complex I. Complex I has an NADH dehydrogenase activity with ubiquinone as an immediate electron acceptor and mediates the transfer of electrons from NADH to the respiratory chain.

See full target information NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:3331-3343 PubMed33707295

2021

Mutations Cause Mechanotransduction Defects in Cochlear Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alix Trouillet,Katharine K Miller,Shefin Sam George,Pei Wang,Noor-E-Seher Ali,Anthony Ricci,Nicolas Grillet

EMBO reports 21:e48833 PubMed31721420

2019

MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Ricarda Richter-Dennerlein,David Pacheu-Grau,Fan Liu,Ying Zhu,Sven Dennerlein,Peter Rehling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFB10 antibody [EPR16230]

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230] (AB192245)

Western blot - Anti-NDUFB10 antibody [EPR16230] (AB192245)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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