Rabbit Recombinant Monoclonal NDUFB10 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ICC/IF | WB | |
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Human | Tested | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Accessory subunit that is involved in the functional assembly of the mitochondrial respiratory chain complex I. Complex I has an NADH dehydrogenase activity with ubiquinone as an immediate electron acceptor and mediates the transfer of electrons from NADH to the respiratory chain.
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, Complex I-PDSW, NADH-ubiquinone oxidoreductase PDSW subunit, CI-PDSW, NDUFB10
Rabbit Recombinant Monoclonal NDUFB10 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab251116 is the carrier-free version of Anti-NDUFB10 antibody [EPR16230] ab192245.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The NDUFB10 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex 10 is a component of the mitochondrial respiratory chain complex I. This protein has an approximate mass of 20 kDa. It is expressed mainly in the mitochondria of eukaryotic cells where it plays an important role in the process of oxidative phosphorylation. NDUFB10 is integral to the inner mitochondrial membrane where it acts as part of the machinery that facilitates electron transfer from NADH to ubiquinone.
NDUFB10 plays a significant role in cellular energy production by being a subunit of complex I in the mitochondrial electron transport chain. As part of this large enzyme complex NDUFB10 contributes to the overall stability and function of complex I facilitating ATP production by driving the conversion of energy derived from nutrients into a usable form. Its presence in the mitochondrial inner membrane highlights its essential role in maintaining efficient energy metabolism and cellular respiration.
The electron transport chain pathway incorporates NDUFB10 as a vital component. This pathway plays a major role in oxidative phosphorylation where NDUFB10 interacts closely with other mitochondrial proteins like NDUFS1 and NDUFA1 within complex I. Besides serving in ATP synthesis these interactions help in maintaining overall cellular bioenergetics and proper mitochondrial function demonstrating the integrated role NDUFB10 plays alongside other proteins in energy metabolism.
Malfunction or mutations in NDUFB10 have been linked to mitochondrial disorders including mitochondrial encephalomyopathy and Leber's hereditary optic neuropathy. These conditions often involve disrupted energy metabolism which is critical for tissues with high energy demands. Associated proteins like NDUFS2 and NDUFV1 which also contribute to the function of complex I can compound the severity of these disorders if mutated underlining the importance of NDUFB10 within the broader context of mitochondrial health and disease.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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This data was developed using Anti-NDUFB10 antibody [EPR16230] ab192245, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NDUFB10 antibody [EPR16230] (Anti-NDUFB10 antibody [EPR16230] ab192245) at 1/5000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: HepG2 cell lysate at 20 µg
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: Human fetal kidney lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
This data was developed using Anti-NDUFB10 antibody [EPR16230] ab192245, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB10 with Anti-NDUFB10 antibody [EPR16230] ab192245 at 1/50 dilution ( 6 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are Anti-NDUFB10 antibody [EPR16230] ab192245 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
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