Rabbit Recombinant Monoclonal NDUFB11 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.
Images
![Western blot - Anti-NDUFB11 antibody [EPR15252] (AB183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--western-blot-img79283.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFB11 antibody [EPR15252] (AB183716)")
![Western blot - Anti-NDUFB11 antibody [EPR15252] (AB183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--western-blot-img106085.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFB11 antibody [EPR15252] (AB183716)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (AB183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24146.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (AB183716)")
![Flow Cytometry (Intracellular) - Anti-NDUFB11 antibody [EPR15252] (AB183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--flow-cytometry-intracellular-img80914.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NDUFB11 antibody [EPR15252] (AB183716)")
Publications
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- eLife 11:2022Tissue-specific mitochondrial HIGD1C promotes oxygen sensitivity in carotid body chemoreceptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Timón-Gómez,Alexandra L Scharr,Nicholas Y Wong,Erwin Ni,Arijit Roy,Min Liu,Julisia Chau,Jack L Lampert,Homza Hireed,Noah S Kim,Masood Jan,Alexander R Gupta,Ryan W Day,James M Gardner,Richard J A Wilson,Antoni Barrientos,Andy J ChangPubMed 36255054
- Cell metabolism 33:531-546.e92021C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Wang,Honghe Liu,Kie Itoh,Sungtaek Oh,Liang Zhao,Daisuke Murata,Hiromi Sesaki,Thomas Hartung,Chan Hyun Na,Jiou WangPubMed 33545050
- Nature communications 11:54692020Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrooke C Wilson et. al.PubMed 33122634
- STAR protocols 1:2020Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Timón-Gómez et. al.PubMed 32995753
- Cell reports 31:1076072020Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Timón-Gómez,Joshua Garlich,Rosemary A Stuart,Cristina Ugalde,Antoni BarrientosPubMed 32375044
- European journal of medical genetics 62:1035722018Diverse phenotype in patients with complex I deficiency due to mutations in NDUFB11.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarit Reinson et. al.PubMed 30423443
- Clinical genetics 93:111-1182017NDUFA9 point mutations cause a variable mitochondrial complex I assembly defect.Applications:WBReactive species:HumanF Baertling,L Sánchez-Caballero,M A M van den Brand,C-W Fung,S H-S Chan,V C-N Wong,D M E Hellebrekers,I F M de Coo,J A M Smeitink,R J T Rodenburg,L G J NijtmansPubMed 28671271
- Nature 538:123-1262016Accessory subunits are integral for assembly and function of human mitochondrial complex I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid A Stroud et. al.PubMed 27626371
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/240 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Alternative names
UNQ111/PRO1064, NDUFB11, Complex I-ESSS, NADH-ubiquinone oxidoreductase ESSS subunit, Neuronal protein 17.3, CI-ESSS, Np17.3, p17.3
Recommended products
Rabbit Recombinant Monoclonal NDUFB11 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15252
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NDUFB11 also known as NADH:ubiquinone oxidoreductase subunit B11 is a component of mitochondrial Complex I. It has a molecular weight of approximately 12 kDa. This protein contributes to the proper function of the electron transport chain. NDUFB11 is expressed mainly in tissues with high energy demands like the heart muscle and brain. It plays a role in facilitating electron transfer from NADH to ubiquinone which is a critical step in cellular respiration.
- Biological function summary
NDUFB11 becomes part of the mitochondrial respiratory chain complex I an essential structure for ATP production through oxidative phosphorylation. This complex includes multiple subunits and is located on the inner mitochondrial membrane. NDUFB11 contributes to the assembly and stability of this complex ensuring efficient energy production within cells. Complex I dysfunction often impacts energy-dependent tissues leading to various metabolic challenges.
- Pathways
NDUFB11 participates in the oxidative phosphorylation pathway vital for energy production in eukaryotic cells. It interacts with other important proteins of the electron transport chain like NDUFS1 and NDUFA13. These proteins collectively ensure the smooth transfer of electrons for ATP synthesis. Additionally NDUFB11 is involved in the mitochondrial electron transport which is essential for maintaining cellular metabolic processes.
- Associated diseases and disorders
NDUFB11 mutations link to mitochondrial diseases such as Leigh syndrome characterized by neurometabolic dysfunction. This protein's malfunction can also connect to complex I deficiency affecting cellular respiration and leading to various clinical symptoms including muscle weakness and neurological impairments. Mitochondrial diseases associated with NDUFB11 may also involve interactions with other mitochondrial subunits indicating an intricate relationship with overall mitochondrial health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--western-blot-img79283.jpg "Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)")
Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
All lanes: Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution
All lanes: Human heart tissue lysate at 20 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 17 kDa
![Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--western-blot-img106085.jpg "Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)")
Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716)
Lanes 1 - 2: Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/50000 dilution
Lane 3: Western blot - Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: HepG2 cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24146.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB11 antibody [EPR15252] (ab183716)
Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling NDUFB11 with ab183716 at a 1/250 dilution. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-NDUFB11 antibody [EPR15252] (ab183716), expandable thumbnail](https://content.abcam.com/products/images/ndufb11-antibody-epr15252-ab183716--flow-cytometry-intracellular-img80914.jpg "Flow Cytometry (Intracellular) - Anti-NDUFB11 antibody [EPR15252] (ab183716)")
Flow Cytometry (Intracellular) - Anti-NDUFB11 antibody [EPR15252] (ab183716)
Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling NDUFB11 with ab183716 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
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