AB186748

Anti-NDUFB2 antibody [EPR15569]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal NDUFB2 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

Complex I-AGGG, NADH-ubiquinone oxidoreductase AGGG subunit, CI-AGGG, NDUFB2

6 Images

Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling NDUFB2 with ab186748 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (AB186748)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFB2 with ab186748 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

All lanes:

Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

All lanes:

Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/10000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

All lanes:

Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/1000 dilution

Lane 1:

293 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

All lanes:

Western blot - Anti-NDUFB2 antibody [EPR15569] (ab186748) at 1/2000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Mouse spleen tissue lysate at 10 µg

Lane 4:

Rat brain tissue lysate at 10 µg

Lane 5:

Rat heart tissue lysate at 10 µg

Lane 6:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

false

Carrier free

Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15569

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFB2 also known as NADH:Ubiquinone Oxidoreductase Subunit B2 is a component of the mitochondrial complex I. This protein has a molecular mass of approximately 12.7 kDa. It primarily expresses in tissues with high energy demands such as the heart and brain due to its role in energy production. NDUFB2 integrates into the mitochondrial membrane where it participates in electron transport for ATP synthesis.

Biological function summary

NDUFB2 acts as a subunit of complex I the first enzyme complex in the mitochondrial electron transport chain. By facilitating the transfer of electrons from NADH to ubiquinone NDUFB2 is essential for initiating the process that leads to the generation of ATP. Complex I itself comprises 44 subunits and NDUFB2 interacts closely with other subunits to perform its function. The protein's activity influences mitochondrial function and the efficiency of ATP production.

Pathways

NDUFB2 plays a pivotal role in mitochondrial oxidative phosphorylation. This pathway underlies cellular respiration which is key to converting the energy stored in nutrients into ATP. NDUFB2's function is closely linked with cytochrome c oxidase a component of complex IV as these complexes sequentially facilitate electron transport and energy conversion. Proper functioning of complex I where NDUFB2 resides is critical for maintaining the proton gradient and overall balance of the oxidative phosphorylation pathway.

Defects in NDUFB2 associate with mitochondrial disorders primarily affecting the nervous system and muscles such as Leigh syndrome. Dysfunctional NDUFB2 impairments lead to deficits in ATP production exacerbating symptoms in these energy-demanding tissues. Moreover the protein's role in oxidative phosphorylation suggests potential interactions with other proteins like succinate dehydrogenase which are involved in energy metabolism disorders increasing the complexity of disease mechanisms associated with mitochondrial dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 56:2588-2601 PubMed39617791

2024

Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Pengpeng Yue,Xiaoyan Lv,Hankun Cao,Yongkang Zou,Jian You,Jun Luo,Zhongshan Lu,Hao Chen,Zhongzhong Liu,Zibiao Zhong,Yan Xiong,Xiaoli Fan,Qifa Ye

Cell 180:1160-1177.e20 PubMed32160526

2020

A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Rui Liang,Emily Lingeman,Thao Luong,Saba Ahmed,Matthias Muhar,Truc Nguyen,James A Olzmann,Jacob E Corn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFB2 antibody [EPR15569]

Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Western blot - Anti-NDUFB2 antibody [EPR15569] (AB186748)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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