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Rabbit Recombinant Monoclonal NDUFB2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (AB250899), expandable thumbnail
  • Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (AB250899), expandable thumbnail
  • Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (AB250899), expandable thumbnail
  • Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (AB250899), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (AB250899), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

Complex I-AGGG, NADH-ubiquinone oxidoreductase AGGG subunit, CI-AGGG, NDUFB2

Recommended products

Rabbit Recombinant Monoclonal NDUFB2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15569
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250899 is the carrier-free version of Anti-NDUFB2 antibody [EPR15569] ab186748.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NDUFB2 also known as NADH:Ubiquinone Oxidoreductase Subunit B2 is a component of the mitochondrial complex I. This protein has a molecular mass of approximately 12.7 kDa. It primarily expresses in tissues with high energy demands such as the heart and brain due to its role in energy production. NDUFB2 integrates into the mitochondrial membrane where it participates in electron transport for ATP synthesis.

Biological function summary

NDUFB2 acts as a subunit of complex I the first enzyme complex in the mitochondrial electron transport chain. By facilitating the transfer of electrons from NADH to ubiquinone NDUFB2 is essential for initiating the process that leads to the generation of ATP. Complex I itself comprises 44 subunits and NDUFB2 interacts closely with other subunits to perform its function. The protein's activity influences mitochondrial function and the efficiency of ATP production.

Pathways

NDUFB2 plays a pivotal role in mitochondrial oxidative phosphorylation. This pathway underlies cellular respiration which is key to converting the energy stored in nutrients into ATP. NDUFB2's function is closely linked with cytochrome c oxidase a component of complex IV as these complexes sequentially facilitate electron transport and energy conversion. Proper functioning of complex I where NDUFB2 resides is critical for maintaining the proton gradient and overall balance of the oxidative phosphorylation pathway.

Associated diseases and disorders

Defects in NDUFB2 associate with mitochondrial disorders primarily affecting the nervous system and muscles such as Leigh syndrome. Dysfunctional NDUFB2 impairments lead to deficits in ATP production exacerbating symptoms in these energy-demanding tissues. Moreover the protein's role in oxidative phosphorylation suggests potential interactions with other proteins like succinate dehydrogenase which are involved in energy metabolism disorders increasing the complexity of disease mechanisms associated with mitochondrial dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899), expandable thumbnail

    Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899)

    This data was developed using Anti-NDUFB2 antibody [EPR15569] ab186748, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFB2 antibody [EPR15569] (Anti-NDUFB2 antibody [EPR15569] ab186748) at 1/10000 dilution

    All lanes: Human fetal heart lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899), expandable thumbnail

    Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899)

    This data was developed using Anti-NDUFB2 antibody [EPR15569] ab186748, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFB2 antibody [EPR15569] (Anti-NDUFB2 antibody [EPR15569] ab186748) at 1/1000 dilution

    Lane 1: 293 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899), expandable thumbnail

    Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899)

    This data was developed using Anti-NDUFB2 antibody [EPR15569] ab186748, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFB2 antibody [EPR15569] (Anti-NDUFB2 antibody [EPR15569] ab186748) at 1/1000 dilution

    Lane 1: Human skeletal muscle tissue lysate at 10 µg

    Lane 2: Human fetal brain tissue lysate at 10 µg

    Lane 3: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 12 kDa

  • Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899), expandable thumbnail

    Western blot - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899)

    This data was developed using Anti-NDUFB2 antibody [EPR15569] ab186748, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFB2 antibody [EPR15569] (Anti-NDUFB2 antibody [EPR15569] ab186748) at 1/2000 dilution

    Lane 1: Mouse heart tissue lysate at 10 µg

    Lane 2: Mouse kidney tissue lysate at 10 µg

    Lane 3: Mouse spleen tissue lysate at 10 µg

    Lane 4: Rat brain tissue lysate at 10 µg

    Lane 5: Rat heart tissue lysate at 10 µg

    Lane 6: Rat kidney tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899)

    This data was developed using Anti-NDUFB2 antibody [EPR15569] ab186748, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFB2 with Anti-NDUFB2 antibody [EPR15569] ab186748 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NDUFB2 antibody [EPR15569] - BSA and Azide free (ab250899)

    This data was developed using Anti-NDUFB2 antibody [EPR15569] ab186748, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling NDUFB2 with Anti-NDUFB2 antibody [EPR15569] ab186748 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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