Anti-NDUFB3 antibody [EPR15571] (ab202585)

Rabbit Recombinant Monoclonal NDUFB3 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-NDUFB3 antibody [EPR15571] (AB202585), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Tested
Rat	Expected	Tested	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/40	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/300	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/60	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information NDUFB3

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3, Complex I-B12, NADH-ubiquinone oxidoreductase B12 subunit, CI-B12, NDUFB3

Recommended products

Rabbit Recombinant Monoclonal NDUFB3 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR15571
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein NDUFB3 also known as NADH:ubiquinone oxidoreductase subunit B3 plays a significant role in cellular respiration. It is a component of the mitochondrial complex I where it participates in the electron transport chain. NDUFB3 is a small protein with a molecular weight of approximately 12 kDa. It is expressed in various human tissues notably in high-energy-demanding organs such as the heart brain and skeletal muscles where mitochondria are abundant.

Biological function summary

NDUFB3 contributes to the transfer of electrons from NADH to ubiquinone an essential step in oxidative phosphorylation. This protein is part of the larger NADH dehydrogenase complex also called complex I which consists of 45 protein subunits. Complex I is the largest enzyme complex in the electron transport chain and serves a fundamental role in ATP production by establishing a proton gradient across the inner mitochondrial membrane.

Pathways

NDUFB3 significantly affects mitochondrial energy metabolism. It is an integral component of the electron transport chain. Through this it participates in pathways such as oxidative phosphorylation and cellular respiration. NDUFB3 associates with other proteins subunits of complex I such as NDUFS2 for electron transport which ultimately powers ATP synthesis by ATP synthase.

Associated diseases and disorders

Disruptions in NDUFB3 function can contribute to mitochondrial diseases particularly those related to energy metabolism. Mutations in this protein have been linked to Leigh syndrome a severe neurological disorder. Moreover the dysfunction of complex I which includes NDUFB3 has connections to Parkinson's disease highlighting the importance of this protein in neurodegenerative conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
Lane 4: Human fetal spleen lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling NDUFB3 with ab202585 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling NDUFB3 with ab202585 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab202585 at 1/300 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Flow Cytometry (Intracellular) - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Intracellular Flow Cytometry analysis of HepG2 cells labelling NDUFB3 (red) with purified ab202585 at dilution of 1/60. The secondary antibody used was Alexa Fluorr^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585) at 1/1000 dilution
All lanes: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Predicted band size: 11 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling NDUFB3 with ab202585 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585) at 1/5000 dilution
All lanes: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3min
Immunoprecipitation - Anti-NDUFB3 antibody [EPR15571] (ab202585)
NDUFB3 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab202585 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab202585 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HepG2 whole cell lysate 10 μg (Input). Lane 2: ab202585 IP in HepG2 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab202585 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
All lanes: Immunoprecipitation - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Predicted band size: 11 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling NDUFB3 with ab202585 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab202585 at 1/300 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NDUFB3 antibody [EPR15571] (ab202585) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 10 µg
Lane 2: Mouse heart tissue lysate at 10 µg
Lane 3: Mouse kidney tissue lysate at 10 µg
Lane 4: Rat brain tissue lysate at 10 µg
Lane 5: Rat heart tissue lysate at 10 µg
Lane 6: Rat kidney tissue lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB3 antibody [EPR15571] (ab202585)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NDUFB3 with ab202585 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat kidney tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.