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AB110243

Anti-NDUFB4 antibody [17G3D9E12]

5

(1 Review)

|

(19 Publications)

Mouse Monoclonal NDUFB4 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Cow, Rat samples. Cited in 19 publications.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, NADH-ubiquinone oxidoreductase B15 subunit, CI-B15, NDUFB4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)
  • IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)

Immunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step : Heat mediated. Permeabilization : No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.

This image is courtesy of Philippa Hepplewhite, University of Newcastle, UK

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)

Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
Cultured Human fibroblasts were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110243 at 15μg/ml, followed by a fluorescent goat-anti-mouse IgG.

Flow Cytometry - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)

HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Western blot - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)
  • WB

Unknown

Western blot - Anti-NDUFB4 antibody [17G3D9E12] (AB110243)

All lanes:

Western blot - Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/mL

Lane 1:

Isolated mitochondria from Human heart at 5 µg

Lane 2:

Isolated mitochondria from cow heart at 1 µg

Lane 3:

Isolated mitochondria from rat heart at 10 µg

Predicted band size: 15 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

17G3D9E12

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Rat, Cow, Human

Applications

ICC/IF, Flow Cyt, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p>(heat-induced antigen-retrieval improves signal).</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-15 µg/mL", "ICCIF-species-notes": "<p>Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.</p>" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Near homogeneity as judged by SDS-PAGE (>95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFB4 also known as NADH: ubiquinone oxidoreductase subunit B4 plays a role in the mitochondrial respiratory chain complex I. Weighing approximately 15 kDa NDUFB4 is expressed in mitochondria across various cell types. It couples electron transfer from NADH to ubiquinone thereby contributing to energy production process in cells. NDUFB4 is important in maintaining mitochondrial functionality due to its role in the electron transport chain.
Biological function summary

NADH: ubiquinone oxidoreductase subunit B4 is part of the NADH dehydrogenase complex I. This complex is involved in the initial step of the mitochondrial electron transport chain. NDUFB4 supports the bioenergetic functions of cells by facilitating redox reactions. It also helps establish the electrochemical gradient needed for ATP synthesis. This protein interacts synergistically with other subunits in complex I to ensure efficient energy conversion.

Pathways

NADH dehydrogenase complex I which includes NDUFB4 is central in oxidative phosphorylation. This process supports energy production by converting NADH and FADH2 into ATP. NDUFB4 is integrally linked with proteins like NDUFS2 and NDUFV1 within this pathway ensuring electron transport is seamless. Complex I contributes to metabolic processes like the TCA cycle by feeding the mitochondria with necessary energy intermediates.

Malfunction of NDUFB4 in complex I links to mitochondrial diseases such as Leigh syndrome. This syndrome affects central nervous system development. Mitochondrial encephalopathies are another group where NDUFB4 involvement is observed. In these conditions proteins like NDUFA1 and mitochondrial DNA mutations can manifest similar neurodegenerative symptoms due to impaired oxidative phosphorylation. Therefore understanding NDUFB4's function helps in identifying therapeutic interventions for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFB4
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:105626 PubMed38211818

2024

Accessory subunit NDUFB4 participates in mitochondrial complex I supercomplex formation.

Applications

Unspecified application

Species

Unspecified reactive species

Gaganvir Parmar,Claire Fong-McMaster,Chantal A Pileggi,David A Patten,Alexanne Cuillerier,Stephanie Myers,Ying Wang,Siegfried Hekimi,Miroslava Cuperlovic-Culf,Mary-Ellen Harper

Frontiers in genetics 14:1190222 PubMed37588046

2023

A novel IBA57 variant is associated with mitochondrial iron-sulfur protein deficiency and necrotizing myelopathy in dogs.

Applications

WB

Species

Dog

Paul J J Mandigers,Oliver Stehling,Manon Vos-Loohuis,Frank G Van Steenbeek,Roland Lill,Peter A Leegwater

Genomics, proteomics & bioinformatics 18:679-695 PubMed33607292

2021

Screening of Potential Biomarkers for Gastric Cancer with Diagnostic Value Using Label-free Global Proteome Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxi Song,Jun Wang,Jingxu Sun,Xiaowan Chen,Jinxin Shi,Zhonghua Wu,Dehao Yu,Fei Zhang,Zhenning Wang

Life science alliance 3: PubMed32788226

2020

MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Ruchika Anand,Arun Kumar Kondadi,Jana Meisterknecht,Mathias Golombek,Oliver Nortmann,Julia Riedel,Leon Peifer-Weiß,Nahal Brocke-Ahmadinejad,David Schlütermann,Björn Stork,Thomas O Eichmann,Ilka Wittig,Andreas S Reichert

Journal of experimental zoology. Part B, Molecular and developmental evolution 334:362-372 PubMed32779369

2020

Signature of high altitude adaptation in the gluteus proteome of the yak.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Wei Xin,Zhi-Xin Chai,Cheng-Fu Zhang,Qiang Zhang,Yong Zhu,Han-Wen Cao,Cidan Yang Ji,Xiao-Ying Chen,Hui Jiang,Jin-Cheng Zhong,Qiu-Mei Ji

Cell 180:1160-1177.e20 PubMed32160526

2020

A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Rui Liang,Emily Lingeman,Thao Luong,Saba Ahmed,Matthias Muhar,Truc Nguyen,James A Olzmann,Jacob E Corn

Cancer cell 33:450-462.e10 PubMed29533785

2018

Systematic Functional Annotation of Somatic Mutations in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Kwok-Shing Ng,Jun Li,Kang Jin Jeong,Shan Shao,Hu Chen,Yiu Huen Tsang,Sohini Sengupta,Zixing Wang,Venkata Hemanjani Bhavana,Richard Tran,Stephanie Soewito,Darlan Conterno Minussi,Daniela Moreno,Kathleen Kong,Turgut Dogruluk,Hengyu Lu,Jianjiong Gao,Collin Tokheim,Daniel Cui Zhou,Amber M Johnson,Jia Zeng,Carman Ka Man Ip,Zhenlin Ju,Matthew Wester,Shuangxing Yu,Yongsheng Li,Christopher P Vellano,Nikolaus Schultz,Rachel Karchin,Li Ding,Yiling Lu,Lydia Wai Ting Cheung,Ken Chen,Kenna R Shaw,Funda Meric-Bernstam,Kenneth L Scott,Song Yi,Nidhi Sahni,Han Liang,Gordon B Mills

PloS one 11:e0160258 PubMed27479602

2016

Mic13 Is Essential for Formation of Crista Junctions in Mammalian Cells.

Applications

WB

Species

Unspecified reactive species

Ruchika Anand,Valentina Strecker,Jennifer Urbach,Ilka Wittig,Andreas S Reichert

Cell death & disease 5:e1540 PubMed25429617

2014

Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.

Applications

IF

Species

Human

H-Y Chang,T-C Huang,N-N Chen,H-C Huang,H-F Juan

Proceedings of the National Academy of Sciences of 110:E3622-30 PubMed24003133

2013

Tissue- and cell-type-specific manifestations of heteroplasmic mtDNA 3243A>G mutation in human induced pluripotent stem cell-derived disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Riikka H Hämäläinen,Tuula Manninen,Hanna Koivumäki,Mikhail Kislin,Timo Otonkoski,Anu Suomalainen
View all publications
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